CAJ | 학술논문

利用M RS培养基从多种蔬菜中，筛选产酸且具有较好抑菌活性的芽胞杆菌．经多次复筛，最终从96株初筛菌株中，获得一株抑菌谱广、抑菌活性高的菌株N5．通过对其形态学、生理生化特征及其16SrDNA D1/D2区域的分析表明，N5为蜡状芽胞杆菌．抑菌特性结果表明，N5产生的抑菌物质存在于发酵液上清中，具有较好的热稳定性和耐酸性．
이용M RS배양기종다충소채중，사선산산차구유교호억균활성적아포간균．경다차복사，최종종96주초사균주중，획득일주억균보엄、억균활성고적균주N5．통과대기형태학、생리생화특정급기16SrDNA D1/D2구역적분석표명，N5위사상아포간균．억균특성결과표명，N5산생적억균물질존재우발효액상청중，구유교호적열은정성화내산성．
Bacillus cereus producing organic acids and exhibiting high antimicrobial activity is isolated from a variety of vegetables by employing MRS medium .After many times of screening ,of the 96 strains ,a strain N5 with broad antimicrobial spectrum and high antimicrobial activity is obtained .The a-nalysis of physiological and biochemical characteristics and 16Sr DNA D1/D2 domain shows that N5 was identified as Bacillus cereus .Furthermore ,the result of its antimicrobial properties shows that antibac-terial substances in the fermentation supernatant have the resistance to acid and the thermal stability .