CAJ | 학술논문

AtT R1是拟南芥中发现的与植物高温和高盐等非生物胁迫相关的全新基因。采用GAL4酵母双杂交系统，以AtTR1作为诱饵蛋白，对拟南芥cDNA 文库进行筛选，获得了与AtTR1有潜在相互作用的蛋白质PATL6（Patellin6）。进一步采用GST pull-down方法在体外验证了PATL6与AtTR1的相互作用。采用Real-time PCR检测 PA TL6在NaCl和Man-nitol处理后的表达情况，结果显示 PAT L6的表达受高盐和高渗的诱导，是一个与非生物胁迫应答相关的基因。这些实验结果为进一步研究AtTR1及其相互作用蛋白质在植物抗逆胁迫中的作用奠定了基础。
AtT R1시의남개중발현적여식물고온화고염등비생물협박상관적전신기인。채용GAL4효모쌍잡교계통，이AtTR1작위유이단백，대의남개cDNA 문고진행사선，획득료여AtTR1유잠재상호작용적단백질PATL6（Patellin6）。진일보채용GST pull-down방법재체외험증료PATL6여AtTR1적상호작용。채용Real-time PCR검측 PA TL6재NaCl화Man-nitol처리후적표체정황，결과현시 PAT L6적표체수고염화고삼적유도，시일개여비생물협박응답상관적기인。저사실험결과위진일보연구AtTR1급기상호작용단백질재식물항역협박중적작용전정료기출。
AtTR1 was a new gene which is relative to high temperature and salt stress .AtTR1 was used as a bait to screen proteins interacting with AtTR1 in A rabidopsis cDNA library by yeast two-hybrid system .PATL6 (Patellin6) which could interact with AtTR1 was obtained .GST pull-down result showed that PATL6 could interact with AtTR1 in vitro .The expression of PA TL6 in A rabidopsis after treated with NaCl and Mannitol was assayed by Real-time PCR .The result showed that the expression level could be induced by high salt and high osmotic stresses and PA TL6 was an abiotic stress response-relate gene .This study provides the foundation for further understanding the functions of these proteins in plant defense responses .