CAJ | 학술논문

构建番茄SlWRKY53的过量表达载体，通过农杆菌介导转入番茄（Solanum lycoper-sicum）品种A ilsa Craig（AC＋），获得转基因阳性植株。定量分析显示，这些转基因株系中 Sl-WRKY53基因表达量显著高于野生型。表型分析显示，转基因植株对盐胁迫抗性强于野生型。对抗病性进行检测，转基因植株抗性稍强，但与野生型相比无明显差异。由此推测番茄 Sl-WRKY53基因的过量表达能够一定程度增强植株抵抗盐胁迫的能力。
구건번가SlWRKY53적과량표체재체，통과농간균개도전입번가（Solanum lycoper-sicum）품충A ilsa Craig（AC＋），획득전기인양성식주。정량분석현시，저사전기인주계중 Sl-WRKY53기인표체량현저고우야생형。표형분석현시，전기인식주대염협박항성강우야생형。대항병성진행검측，전기인식주항성초강，단여야생형상비무명현차이。유차추측번가 Sl-WRKY53기인적과량표체능구일정정도증강식주저항염협박적능력。
The overexpression vector containing the full-length coding region of SlWRKY53 gene was constructed and introduced into tomato cv .Ailsa Craig by A grobacterium-mediated genetic transforma-tion .Real-time PCR revealed a significant increase of SlWRKY53 expression levels in the transgenic lines compared with that of wild-type plants .Further study indicated that these transgenic lines dis-played enhanced tolerance to NaCl treatment .In addition ,the transgenic lines were shown slightly en-hanced resistance to Pst DC3000 pathogen inoculation than that of wild-type plants but without signifi-cant difference .Thus ,up-regulation of SlWRKY53 in tomato plants might result in enhanced tolerance to salt stress .