数理医药学杂志
數理醫藥學雜誌
수리의약학잡지
JOURNAL OF MATHEMATICAL MEDICINE
2013年
6期
660-662
,共3页
屠小明%罗建平%吴海玮
屠小明%囉建平%吳海瑋
도소명%라건평%오해위
参数估计%标准化%拟合优度%非线性最小二乘
參數估計%標準化%擬閤優度%非線性最小二乘
삼수고계%표준화%의합우도%비선성최소이승
parameter estimation%standardization%goodness of fit%nonlinear least square
探讨了在 ELISA 法检测结果标准化过程中,不同参数估计方法对标准化结果的影响。标准化方法采用改良光密度值标准化变换(I-STOD),标准化公式中的参数估计分别采用线性最小二乘法和非线性最小二乘中的高斯牛顿迭代法。结果显示线性最小二乘估计所得到的标准曲线主要由浓度较低的几个点所决定,而非线性最小二乘所确定的标准曲线总体上更接近实验数据,真实体现了不同实验板的反应动力学特性。非线性最小二乘更适用于标准化过程中的参数估计,有效降低了检测结果在板间的变异。
探討瞭在 ELISA 法檢測結果標準化過程中,不同參數估計方法對標準化結果的影響。標準化方法採用改良光密度值標準化變換(I-STOD),標準化公式中的參數估計分彆採用線性最小二乘法和非線性最小二乘中的高斯牛頓迭代法。結果顯示線性最小二乘估計所得到的標準麯線主要由濃度較低的幾箇點所決定,而非線性最小二乘所確定的標準麯線總體上更接近實驗數據,真實體現瞭不同實驗闆的反應動力學特性。非線性最小二乘更適用于標準化過程中的參數估計,有效降低瞭檢測結果在闆間的變異。
탐토료재 ELISA 법검측결과표준화과정중,불동삼수고계방법대표준화결과적영향。표준화방법채용개량광밀도치표준화변환(I-STOD),표준화공식중적삼수고계분별채용선성최소이승법화비선성최소이승중적고사우돈질대법。결과현시선성최소이승고계소득도적표준곡선주요유농도교저적궤개점소결정,이비선성최소이승소학정적표준곡선총체상경접근실험수거,진실체현료불동실험판적반응동역학특성。비선성최소이승경괄용우표준화과정중적삼수고계,유효강저료검측결과재판간적변이。
The impact of different parameter estimation methods on standardized results was explored during standardization of testing results from ELISA .'Improved Standardization method for Optical Density'(I-STOD) was applied to realize standardization .Linear least square (LLS) and Gauss-Newton iteration from nonlinear least square (NLS) were used to estimate parameter in I-STOD formula .Results indicated that cal-ibration curve generated by LLS was lagrely depended on the several initial points .Calibration curve genera-ted by NLS was more close to real data .It was a better representation of what reacted on experimental plates .Therefore ,NLS was more appropriately for parameter estimation in standardization of ELISA testing results and variation between plates was effectively decreased .