全科医学临床与教育
全科醫學臨床與教育
전과의학림상여교육
CLINICAL EDUCATION OF GENERAL PRACTICE
2013年
6期
608-611
,共4页
移动克雷伯菌%β-内酰胺酶%聚合酶链式反应
移動剋雷伯菌%β-內酰胺酶%聚閤酶鏈式反應
이동극뢰백균%β-내선알매%취합매련식반응
klebsiella mobile%beta laclamases%polymerase chain reaction
目的:研究移动克雷伯菌临床分离株β-内酰胺酶编码基因。方法采用VITEK2-compact分析系统的药敏卡AST-GN13及纸片扩散法(K-B法)测定抗菌药物的敏感性,聚合酶链反应检测24株耐药移动克雷伯菌A~D四类26种β-内酰胺酶编码基因检测。结果24株耐药移动克雷伯菌对碳青霉烯类药物有较高的敏感性,但对第四、第三、第二代β-内酰胺类药物的敏感性呈梯度下降。 A类β-内酰胺酶编码基因检出TEM、SHV、CTX-M-1,阳性率分别为100%、12.50%、8.33%。B~D三类β-内酰胺酶编码基因没有检出。转座子之转座酶与TEM型β-内酰胺酶基因编码基因(tnp-TEM)连锁检测阳性4株,阳性率16.70%。结论 TEM+CTX-M-1群与TEM+SHV双重阳性可能会加重对β-内酰胺类药物的耐药程度,tnp-TEM连锁检测阳性提示TEM基因可高表达而加重对β-内酰胺类药物的耐药程度。
目的:研究移動剋雷伯菌臨床分離株β-內酰胺酶編碼基因。方法採用VITEK2-compact分析繫統的藥敏卡AST-GN13及紙片擴散法(K-B法)測定抗菌藥物的敏感性,聚閤酶鏈反應檢測24株耐藥移動剋雷伯菌A~D四類26種β-內酰胺酶編碼基因檢測。結果24株耐藥移動剋雷伯菌對碳青黴烯類藥物有較高的敏感性,但對第四、第三、第二代β-內酰胺類藥物的敏感性呈梯度下降。 A類β-內酰胺酶編碼基因檢齣TEM、SHV、CTX-M-1,暘性率分彆為100%、12.50%、8.33%。B~D三類β-內酰胺酶編碼基因沒有檢齣。轉座子之轉座酶與TEM型β-內酰胺酶基因編碼基因(tnp-TEM)連鎖檢測暘性4株,暘性率16.70%。結論 TEM+CTX-M-1群與TEM+SHV雙重暘性可能會加重對β-內酰胺類藥物的耐藥程度,tnp-TEM連鎖檢測暘性提示TEM基因可高錶達而加重對β-內酰胺類藥物的耐藥程度。
목적:연구이동극뢰백균림상분리주β-내선알매편마기인。방법채용VITEK2-compact분석계통적약민잡AST-GN13급지편확산법(K-B법)측정항균약물적민감성,취합매련반응검측24주내약이동극뢰백균A~D사류26충β-내선알매편마기인검측。결과24주내약이동극뢰백균대탄청매희류약물유교고적민감성,단대제사、제삼、제이대β-내선알류약물적민감성정제도하강。 A류β-내선알매편마기인검출TEM、SHV、CTX-M-1,양성솔분별위100%、12.50%、8.33%。B~D삼류β-내선알매편마기인몰유검출。전좌자지전좌매여TEM형β-내선알매기인편마기인(tnp-TEM)련쇄검측양성4주,양성솔16.70%。결론 TEM+CTX-M-1군여TEM+SHV쌍중양성가능회가중대β-내선알류약물적내약정도,tnp-TEM련쇄검측양성제시TEM기인가고표체이가중대β-내선알류약물적내약정도。
Objective To determine the sequence of gene for encodingβ-lactamase of Klebsiella mobile strains. Meth-ods VITEK2-compact sensitivity analysis system AST-GN13 and K-B tests were performed to detect antimicrobial sus-ceptibility of the 24 Klebsiella mobile strains, and PCR was used to detect the genetic sequences of the 26 kinds of β-lactamases of the strains. Results The 24 Klebsiella mobilis strains were highly sensitive to carbapenems, but the sensi-tivity to the fourth, third, second generation of Cephalosporin showed a gradient descent. In gene of class Aβ-lactamase, TEM, SHV and CTX-M-1 were detected and their positive rates were 100%, 12.50%, 8.33% respectively. The genes of Class B ~ D β- lactamases were not detected. Both transposase of transposon and tnp-TEM (TEM type beta-lactamase genes) were detected in 4 strains, the positive rate was 16.70%. Conclusion The TEM + CTX-M-1 group and TEM +SHV were positive simultaneously may aggravate the degree of resistance to β-lactam drugs. If tnp-TEM linkage detection was positive, TEM gene may have a high expression so that may aggravate the degree of resistance toβ-lactam drugs.