华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2013年
6期
619-623
,共5页
龋齿%乳牙%牙菌斑%变异链球菌%实时荧光定量聚合酶链反应
齲齒%乳牙%牙菌斑%變異鏈毬菌%實時熒光定量聚閤酶鏈反應
우치%유아%아균반%변이련구균%실시형광정량취합매련반응
dental caries%deciduous tooth%dental plaque%Streptococcus mutans%real-time fluorescence quantita-tive polymerase chain reaction
目的：比较不同龋敏感儿童口腔菌斑中变异链球菌数量及其在菌群中比例的差异。方法采集26名3~4岁不同龋敏感的儿童牙面菌斑，运用TaqMan探针实时荧光定量聚合酶链反应（PCR）检测有龋组和无龋组儿童菌斑中变异链球菌和总菌数量，以及变异链球菌在总菌群中所占的比例，并对结果进行分析比较。结果有龋组和无龋组儿童每毫克菌斑中变异链球菌菌落数分别为1.33×105、1.16×103 CFU·mg-1，二者间差异有统计学意义（P=0.033）；每毫克干重菌斑中总菌落数分别为7.17×107、1.01×108 CFU·mg-1，二者间差异无统计学意义（P=0.418）；有龋组和无龋组变异链球菌在总菌中所占比例分别为0.058 6和0.018 6，二者间差异有统计学意义（P=0.008）。结论无龋与患龋儿童牙面总菌群数量差异无显著性，但患龋儿童牙面菌斑中变异链球菌数量更多，在总菌群中所占比例更大。提示菌斑中变异链球菌与总菌的比例与儿童患龋风险密切相关，可以作为评估龋易感性和预测龋病发展趋势的新指标。
目的：比較不同齲敏感兒童口腔菌斑中變異鏈毬菌數量及其在菌群中比例的差異。方法採集26名3~4歲不同齲敏感的兒童牙麵菌斑，運用TaqMan探針實時熒光定量聚閤酶鏈反應（PCR）檢測有齲組和無齲組兒童菌斑中變異鏈毬菌和總菌數量，以及變異鏈毬菌在總菌群中所佔的比例，併對結果進行分析比較。結果有齲組和無齲組兒童每毫剋菌斑中變異鏈毬菌菌落數分彆為1.33×105、1.16×103 CFU·mg-1，二者間差異有統計學意義（P=0.033）；每毫剋榦重菌斑中總菌落數分彆為7.17×107、1.01×108 CFU·mg-1，二者間差異無統計學意義（P=0.418）；有齲組和無齲組變異鏈毬菌在總菌中所佔比例分彆為0.058 6和0.018 6，二者間差異有統計學意義（P=0.008）。結論無齲與患齲兒童牙麵總菌群數量差異無顯著性，但患齲兒童牙麵菌斑中變異鏈毬菌數量更多，在總菌群中所佔比例更大。提示菌斑中變異鏈毬菌與總菌的比例與兒童患齲風險密切相關，可以作為評估齲易感性和預測齲病髮展趨勢的新指標。
목적：비교불동우민감인동구강균반중변이련구균수량급기재균군중비례적차이。방법채집26명3~4세불동우민감적인동아면균반，운용TaqMan탐침실시형광정량취합매련반응（PCR）검측유우조화무우조인동균반중변이련구균화총균수량，이급변이련구균재총균군중소점적비례，병대결과진행분석비교。결과유우조화무우조인동매호극균반중변이련구균균락수분별위1.33×105、1.16×103 CFU·mg-1，이자간차이유통계학의의（P=0.033）；매호극간중균반중총균락수분별위7.17×107、1.01×108 CFU·mg-1，이자간차이무통계학의의（P=0.418）；유우조화무우조변이련구균재총균중소점비례분별위0.058 6화0.018 6，이자간차이유통계학의의（P=0.008）。결론무우여환우인동아면총균군수량차이무현저성，단환우인동아면균반중변이련구균수량경다，재총균군중소점비례경대。제시균반중변이련구균여총균적비례여인동환우풍험밀절상관，가이작위평고우역감성화예측우병발전추세적신지표。
Objective To determine the quantity of Streptococcus mutans (S. mutans) and the ratio of S. mutans to total bacteria in the dental plaque of different caries-susceptible children. Methods Dental plaque samples from 26 children aged 3 years old to 4 years old were collected. The quantities of S. mutans and total bacteria were determined by TaqMan real-time fluorescence quantitative polymerase chain reaction (PCR). The ratio of S. mutans to total bacteria in children with and without caries was calculated and statistically analyzed. Results In children with and without caries, the quantities of S. mutans were 1.33×105 and 1.16×103 CFU·mg-1, the total bacteria were 7.17×107 and 1.01×108 CFU·mg-1, and the ratios of S. mutans to total bacteria were 0.058 6 and 0.018 6, respectively. Significant differences were observed in the quantities of S. mutans and the total bacteria as well as in the ratios of S. mutans to total bacteria of the two groups (P=0.033, 0.418, 0.008). Conclusion The quantities of total bacteria of the two groups show negligible difference. However, the quantity of S. mutans and the ratio of S. mutans to total bacteria in caries-susceptible children are higher than those in caries-free children. Therefore, the ratios of S. mutans to total bacteria in plaque are closely associated with the prevalence of dental caries in children.
