华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2013年
6期
615-618
,共4页
袁海波%金晶%许春姣%邱喜丽%王璐%吕逢源%刘婷婷%吴颖芳%尹晓敏%方厂云%阙国鹰%彭解英
袁海波%金晶%許春姣%邱喜麗%王璐%呂逢源%劉婷婷%吳穎芳%尹曉敏%方廠雲%闕國鷹%彭解英
원해파%금정%허춘교%구희려%왕로%려봉원%류정정%오영방%윤효민%방엄운%궐국응%팽해영
慢性牙周炎%龈沟液%分泌型卷曲相关蛋白1
慢性牙週炎%齦溝液%分泌型捲麯相關蛋白1
만성아주염%간구액%분비형권곡상관단백1
chronic periodontitis%gingival crevicular fluid%secreted frizzled-related protein 1
目的： 检测分泌型卷曲相关蛋白1（SFRP1）在慢性牙周炎（CP）患者和牙周健康者的表达情况，探讨SFRP1在CP的发生与发展过程中的作用。方法 选择CP患者28例为试验组，按临床附着丧失（CAL）程度分为轻、中、重度3组，另外选择牙周健康者10例为对照组。收集两组研究对象的龈沟液，采用酶联免疫吸附法测定龈沟液中SFRP1的质量浓度。取试验组中22例CP患者的病变牙龈组织标本，以及10例对照组的正常牙龈组织，进行SFRP1免疫组织化学染色，观察着色强度，分析SFRP1表达与不同程度CP的相关性。结果 1）试验组和对照组龈沟液中SFRP1的质量浓度分别为（281.07±33.37）和（245.30±35.69）ng·L-1，试验组明显高于对照组（P<0.05）；龈沟液内SFRP1质量浓度与CAL呈明显正相关（r=0.651，P<0.001）。2）试验组SFRP1着色强度积分值（4.500±0.913）明显高于对照组（2.800±1.135）（P<0.001）；试验组中，轻、中、重度CP组间的着色强度积分值无明显差异（P>0.05）。结论 SFRP1在CP患者龈沟液及牙龈组织中的表达高于牙周健康者，SFRP1可能参与了CP的发生发展过程。
目的： 檢測分泌型捲麯相關蛋白1（SFRP1）在慢性牙週炎（CP）患者和牙週健康者的錶達情況，探討SFRP1在CP的髮生與髮展過程中的作用。方法 選擇CP患者28例為試驗組，按臨床附著喪失（CAL）程度分為輕、中、重度3組，另外選擇牙週健康者10例為對照組。收集兩組研究對象的齦溝液，採用酶聯免疫吸附法測定齦溝液中SFRP1的質量濃度。取試驗組中22例CP患者的病變牙齦組織標本，以及10例對照組的正常牙齦組織，進行SFRP1免疫組織化學染色，觀察著色彊度，分析SFRP1錶達與不同程度CP的相關性。結果 1）試驗組和對照組齦溝液中SFRP1的質量濃度分彆為（281.07±33.37）和（245.30±35.69）ng·L-1，試驗組明顯高于對照組（P<0.05）；齦溝液內SFRP1質量濃度與CAL呈明顯正相關（r=0.651，P<0.001）。2）試驗組SFRP1著色彊度積分值（4.500±0.913）明顯高于對照組（2.800±1.135）（P<0.001）；試驗組中，輕、中、重度CP組間的著色彊度積分值無明顯差異（P>0.05）。結論 SFRP1在CP患者齦溝液及牙齦組織中的錶達高于牙週健康者，SFRP1可能參與瞭CP的髮生髮展過程。
목적： 검측분비형권곡상관단백1（SFRP1）재만성아주염（CP）환자화아주건강자적표체정황，탐토SFRP1재CP적발생여발전과정중적작용。방법 선택CP환자28례위시험조，안림상부착상실（CAL）정도분위경、중、중도3조，령외선택아주건강자10례위대조조。수집량조연구대상적간구액，채용매련면역흡부법측정간구액중SFRP1적질량농도。취시험조중22례CP환자적병변아간조직표본，이급10례대조조적정상아간조직，진행SFRP1면역조직화학염색，관찰착색강도，분석SFRP1표체여불동정도CP적상관성。결과 1）시험조화대조조간구액중SFRP1적질량농도분별위（281.07±33.37）화（245.30±35.69）ng·L-1，시험조명현고우대조조（P<0.05）；간구액내SFRP1질량농도여CAL정명현정상관（r=0.651，P<0.001）。2）시험조SFRP1착색강도적분치（4.500±0.913）명현고우대조조（2.800±1.135）（P<0.001）；시험조중，경、중、중도CP조간적착색강도적분치무명현차이（P>0.05）。결론 SFRP1재CP환자간구액급아간조직중적표체고우아주건강자，SFRP1가능삼여료CP적발생발전과정。
Objective This study detects the expression of secreted frizzled-related protein 1 (SFRP1) in healthy patients and patients with chronic periodontitis (CP) and explores the relationship between SFRP1 and the occurrence and development of CP. Methods First, 28 patients forming the CP group were further divided into mild, moderate, and severe CP subgroups according to clinical attachment loss (CAL) data. Ten healthy volunteers were recruited in the control group. Gingival crevi-cular fluid (GCF) was collected from all of the patients, and the concentration of SFRP1 in the GCF samples was detected using enzyme-linked immunosorbent assay. Next, gingival lesions were obtained from 22 patients in the CP group and healthy gingival tissues were obtained from the 10 healthy patients in the control group. Immunohistochemical analysis for SFRP1 was used to analyze the correlation between the expression of SFRP1 and the severity of CP based on staining intensities. Results The concentration of SFRP1 in GCF samples taken from of the CP group (281.07 ng·L-1±33.37 ng·L-1) was signifi-cantly higher than that in samples taken from the control group (245.30 ng·L-1±35.69 ng·L-1) (P<0.05). A significant positive correlation was observed between the concentration of SFRP1 in GCF and CAL (r=0.651, P<0.001). Furthermore, the SFRP1 scores in the CP groups (4.500±0.913) were significantly higher than those in the control group (2.800±1.135) (P<0.001). SFRP1 scores did not vary significantly among the CP subgroups (P>0.05). Conclusion SFRP1 expression in the CP groups was significantly higher than that in the control group. Thus, SFRP1 may play a significant role in the development of CP.
