CAJ | 학술논문

目的：对检出TPOX基因座三带型进行初步分析。方法：通过PCR扩增，非变性聚丙烯酰胺凝胶电泳及银染技术对TPOX基因座进行分型，并对检出的三带型进行分析。结果：在2例样本中，TPOX基因座均出现三带型等位基因，两者的3个等位基因在荧光检测引物区内除重复次数不同其他序列完全相同。2个样品的等位基因在荧光引物外侧存在几处突变。结论：通过不同方法验证，三带型等位基因在TPOX基因座上确存在。
목적：대검출TPOX기인좌삼대형진행초보분석。방법：통과PCR확증，비변성취병희선알응효전영급은염기술대TPOX기인좌진행분형，병대검출적삼대형진행분석。결과：재2례양본중，TPOX기인좌균출현삼대형등위기인，량자적3개등위기인재형광검측인물구내제중복차수불동기타서렬완전상동。2개양품적등위기인재형광인물외측존재궤처돌변。결론：통과불동방법험증，삼대형등위기인재TPOX기인좌상학존재。
Objective: To investigate the three-banded alleles of the STR TPOX locus. Methods: The genetype of the TPOX locus was determined and analysed by PCR technique followed by the non-denatured PAGE and silver staining. Results:Three-banded alleles were detected in the TPOX locus of two samples. Sequencing proved that the only difference among them was the number of repeat unit for sample one. It might be due to the unequal exchange between homologue chromo-somes, which resulted in the chimera. Conclusion: Three-banded alleles of the TPOX locus were detected and confirmed by different methods.