CAJ | 학술논문

本研究利用SSR标记技术筛选出合适的核心引物，对70个白菜品种进行DNA指纹分析，构建白菜SSR指纹图谱数据库。首先从500对引物中筛选出80对具有多态性的引物，再从其中选取20对多态性高，稳定性好且均匀分布在白菜10条染色体上的核心引物进行PCR扩增，引物多态信息含量(polymorphism information content, PIC)平均为0.716，变化范围0.473~0.932，平均杂合率0.5044。经PCR电泳检测统计得到的指纹图谱进行综合分析，形成各个白菜品种的SSR指纹代码，并且采用ClusterProject对构建的指纹图谱数据进行分析整理，得到各品种的指纹模式图谱，进而分析得到20个核心引物组合可以将70个白菜品种完全分开，并最终建立了白菜品种的SSR指纹图谱数据库。本研究构建的白菜品种SSR指纹图谱数据库，为建立白菜品种特异性、一致性及纯度的鉴定技术体系提供了基础依据。
본연구이용SSR표기기술사선출합괄적핵심인물，대70개백채품충진행DNA지문분석，구건백채SSR지문도보수거고。수선종500대인물중사선출80대구유다태성적인물，재종기중선취20대다태성고，은정성호차균균분포재백채10조염색체상적핵심인물진행PCR확증，인물다태신식함량(polymorphism information content, PIC)평균위0.716，변화범위0.473~0.932，평균잡합솔0.5044。경PCR전영검측통계득도적지문도보진행종합분석，형성각개백채품충적SSR지문대마，병차채용ClusterProject대구건적지문도보수거진행분석정리，득도각품충적지문모식도보，진이분석득도20개핵심인물조합가이장70개백채품충완전분개，병최종건립료백채품충적SSR지문도보수거고。본연구구건적백채품충SSR지문도보수거고，위건립백채품충특이성、일치성급순도적감정기술체계제공료기출의거。
In order to construct SSR fingerprint database of Chinese cabbage varieties, the SSR marker technique was employed to screen suitable core SSR primers to detect SSR fingerprints of 70 Chinese cabbage varieties used in this research. First of all, eighty pairs of SSR with polymorphism among tested varieties were selected from of the used 500 pairs of SSR primers, and then twenty of the selected 80 core primers that exhibit high polym-orphism, good stability and evenly distributed in the ten chromosomes of genome were further selected to perform PCR amplification PCR analysis showed that Polymorphism Information Content (PIC) of the 20 core primers is 0.716 in average with ranges from 0.473 to 0.932 and that the Heterozygous ratio of the 20 core primers is 0.504 4 in average as well. A comprehensive statistic analysis was conducted based on the fingerprints obtained by PCR electrophoresis, which formed SSR fingerprint code of each tested Chinese cabbage varieties. Furthermore the fingerprint data were sorted out by ClusterProject to generate the fingerprint profile of each variety. The results showed that using the twenty core primers can easily distinguish the tested 70 Chinese cabbage varieties, and eventually the database based on SSR fin gerprint for Chinese cabbage varieties was established in this research.The constructed S SR fingerprint database of the Chinese cabbage variety would provide a basis on establis-hment of identification technology for Chinese cabbage variety specificity, consistency and purity.