CAJ | 학술논문

花青素是植物颜色形成的重要成分之一。不同红掌品种中的花青素含量与佛焰苞颜色密切相关。花青素还原酶(ANR)是植物花青素调控代谢途径中催化产生原花青素的一个重要基因。为了探明ANR对花青素合成的作用，本研究根据从抑制差减文库中获得的基因片段，采用RACE技术克隆红掌ANR基因cDNA全长序列。BLAST结果显示，红掌ANR基因全长核苷酸序列与已报道的植物(葡萄，茶树，毛果杨等)同源性为72%~75%，氨基酸序列的同源性则达到78%~79%。RT-PCR结果表明ANR在不同颜色的红掌佛焰苞中表达有差异，其表达量与花青素含量成负相关。
화청소시식물안색형성적중요성분지일。불동홍장품충중적화청소함량여불염포안색밀절상관。화청소환원매(ANR)시식물화청소조공대사도경중최화산생원화청소적일개중요기인。위료탐명ANR대화청소합성적작용，본연구근거종억제차감문고중획득적기인편단，채용RACE기술극륭홍장ANR기인cDNA전장서렬。BLAST결과현시，홍장ANR기인전장핵감산서렬여이보도적식물(포도，다수，모과양등)동원성위72%~75%，안기산서렬적동원성칙체도78%~79%。RT-PCR결과표명ANR재불동안색적홍장불염포중표체유차이，기표체량여화청소함량성부상관。
Anthocyanidin is an important content for plant color appearence. Caltivars of A nthurium andraeanum display varies spathe colors have different concentrations of anthocyanidin. In anthocyanin biosynthetic pathway, Anthocyanidin Reductase (ANR) is a key enzyme to catalyze anthocyanidins to proanthocyanidin (PC). In order to verify the regulation of ANR in anthocyanin synthesis, we cloned the whole cDNA sequence of ANR by RACE technology according to the known ANR fragment from SSH library. BLAST results showed that A nA NR had high similarity, 72%~75%, with reported ANR gene from Populus trichocarpa, Camellia sinensis and V itis vinifera. RT-PCR results showed that in anthurium cultivars with different spathe colors, the expression level of A nA NR was negatively related with the accumulation of anthocyanidin.