CAJ | 학술논문

覆盖在植物表面的蜡粉主要是由特长链饱和脂肪酸的衍生物组成的，它具有防止非气孔的水分散失、病虫害的侵入和太阳辐射的生物学功能。本研究以大白菜花茎上有蜡粉的“08A161”为母本，无蜡粉的“08A235-2”为父本构建了F2代定位群体。遗传分析表明，大白菜花茎有蜡粉性状受单一的显性基因控制，利用308个F2代隐性纯合单株，将蜡粉基因定位在A01连锁群上，根据锚定标记nia-m086a开发SSR引物，初步将目的基因定位在SSRzx45与SSRzx54标记之间，遗传距离分别为1.54 cM和0.56 cM。蜡粉基因的初步定位奠定了基因的精细定位和基因克隆的前期理论基础。
복개재식물표면적사분주요시유특장련포화지방산적연생물조성적，타구유방지비기공적수분산실、병충해적침입화태양복사적생물학공능。본연구이대백채화경상유사분적“08A161”위모본，무사분적“08A235-2”위부본구건료F2대정위군체。유전분석표명，대백채화경유사분성상수단일적현성기인공제，이용308개F2대은성순합단주，장사분기인정위재A01련쇄군상，근거묘정표기nia-m086a개발SSR인물，초보장목적기인정위재SSRzx45여SSRzx54표기지간，유전거리분별위1.54 cM화0.56 cM。사분기인적초보정위전정료기인적정세정위화기인극륭적전기이론기출。
The surface of plants is covered with a cuticular wax, which contains a mixture of very long-chain fatty acid derivatives. This wax layer provides a hydrophobic barrier which reduces non-stomatal water loss, prevents pathogen attack and protects plants from ultraviolet radiation. In this study, we constructed F2 segregating popu-lation taking Chinese cabbage inbred lines‘08A161’(waxy) and‘08A235-2’(glossy) as paternal plants. Genetic analysis indicated that the waxy trait of stem was controlled by a single dominant gene. Using 308 F2 recessive glossy individuals, we mapped the wax gene to A01 of Brassica rapa linkage group and developed SSR markers according nia-m086a as anchored markers, the wax gene was mapped between SSRzx45 and SSRzx54, with a genetic distance of 1.54 cM and 0.56 cM, respectively. The preliminary mapping of wax gene would lay a basis for its fine mapping and gene cloning.