CAJ | 학술논문

分析辣椒属ITS序列，为辣椒属系统位置、进化关系提供分子生物学依据。以辣椒属(Capsicum)4个栽培种36份不同基因型辣椒种质资源为试材，对其rDNA转录间隔区(internal transcribed spacers, ITS)进行PCR扩增。扩增产物经纯化、克隆和测序后，获得ITS序列信息，随后利用MEGA 5.05软件分析其序列特征，并探讨了辣椒属种质资源的系统进化关系。结果表明，辣椒属ITS基本序列全长656 bp，G+C含量57.47%。供试材料的ITS序列长度变异较大，ITS1、5.8S和ITS2区长度变异都有发生。序列比对表明，供试辣椒材料ITS区共有387个变异位点(ITS1：153；5.8S：82；ITS2：152)，信息位点322个，同源性为71.0%~100%，遗传分歧为0~0.3604。以烟草为外类群，将ITS1、5.8S和ITS2序列作为一个共同的数据矩阵进行系统发育树构建，辣椒属C. frutescens和C. baccatum种被单独分出，C. chinense和C. annuum种聚在一起，C. annuum与其他3个栽培种的亲缘关系由近至远分别是C. chinense、C. frutescens和C. baccatum，C. annuum种的32份供试材料主要分为3个分支6个亚分支，聚类分析结果与形态分类不能完全对应。
분석랄초속ITS서렬，위랄초속계통위치、진화관계제공분자생물학의거。이랄초속(Capsicum)4개재배충36빈불동기인형랄초충질자원위시재，대기rDNA전록간격구(internal transcribed spacers, ITS)진행PCR확증。확증산물경순화、극륭화측서후，획득ITS서렬신식，수후이용MEGA 5.05연건분석기서렬특정，병탐토료랄초속충질자원적계통진화관계。결과표명，랄초속ITS기본서렬전장656 bp，G+C함량57.47%。공시재료적ITS서렬장도변이교대，ITS1、5.8S화ITS2구장도변이도유발생。서렬비대표명，공시랄초재료ITS구공유387개변이위점(ITS1：153；5.8S：82；ITS2：152)，신식위점322개，동원성위71.0%~100%，유전분기위0~0.3604。이연초위외류군，장ITS1、5.8S화ITS2서렬작위일개공동적수거구진진행계통발육수구건，랄초속C. frutescens화C. baccatum충피단독분출，C. chinense화C. annuum충취재일기，C. annuum여기타3개재배충적친연관계유근지원분별시C. chinense、C. frutescens화C. baccatum，C. annuum충적32빈공시재료주요분위3개분지6개아분지，취류분석결과여형태분류불능완전대응。
The phylogeny of pepper (Capsicum) was investigated by using ITS sequences and it will provide useful information for pepper classification and evolution. Thirty-six different genotype germplasm resources from four cultivated species in Capsicum were used as materials to amplify the ribosomal DNA internal transcribed spacer (ITS). The amplified fragments were purified to be cloned and sequenced. Then sequences character and phylog-enetics relationship were analyzed using MEGA 5.05 software. The Results showed that the length of Capsicum ITS sequences was 656bp, and its G+C content was 57.47%. There have a signification variation of the length of ITS in these materials, it occurred in ITS1, 5.8S and ITS2. It has 387 variable sites in total (thereinto, ITS1:153;5.8S:82; ITS2: 152), and 322 parsim-info sites. The homology and genetic distances ranged from 71% to 100% and from 0 to 0.360 4, respectively. Using Nicotiana tabacum as outgroup, ITS1, 5.8S and ITS2 sequences as a common data matrix was used to construct of phylogenetic tree. C. frutescens and C. baccatum were divided firstly;C. chinense and C. annuum were clustered into one group. The results from cluster analysis showed that the genetic distances of C. annuum with other three species beginning with nearer were C. chinense, C. frutescens and C. baccatum. Three groups and six sub-groups were clustered by alignment analyses using the 32 accessions of C. annuum, and clustered results did not fully correspon d to the cur rent morphologic classification, suggestion that the genetic differences among those accessions of C. annuum could not be precisely reflected by the current taxonomic system.