CAJ | 학술논문

甜菜是中国重要的糖料作物，为提高甜菜氮素利用效率，对其进行了高亲和铵转运蛋白基因的遗传转化。本研究主要对转A tA MT1；1基因甜菜T1代植株进行了PCR、RT-PCR检测和相关生理特性的分析。结果表明，PCR和RT-PCR检测的T1代转基因甜菜呈阳性，并在根中表达；在控制氮素供应的条件下，转基因植株的叶绿素和氨态氮含量显著高于野生型甜菜，块根产量和含糖率也高于对照，初步证明转A tA MT1；1基因甜菜可提高氮素的利用效率。
첨채시중국중요적당료작물，위제고첨채담소이용효솔，대기진행료고친화안전운단백기인적유전전화。본연구주요대전A tA MT1；1기인첨채T1대식주진행료PCR、RT-PCR검측화상관생리특성적분석。결과표명，PCR화RT-PCR검측적T1대전기인첨채정양성，병재근중표체；재공제담소공응적조건하，전기인식주적협록소화안태담함량현저고우야생형첨채，괴근산량화함당솔야고우대조，초보증명전A tA MT1；1기인첨채가제고담소적이용효솔。
Sugar beet is an important sugar crop, to improve beet nitrogen use efficiency, its genetic transform-ation of the high-affinity ammonium transporter gene was done. This experiment takes a molecule detection and physiological characteristics analysis to the T1 generation of A tA MT1;1 transgenic sugar beet. The results show that the PCR detection and RT-PCR detection of T1 generation of transgenic sugar beet is positive. In the control of nitrogen supply conditions, transgenic plants chlorophyll content and ammonia nitrogen content enhance than the control of wild-type sugar beet, and the difference was significant. Transgenic sugar beet yield and sugar content higher than the wild type, preliminary evidence A tA MT1;1 transgenic sugar beet can increase the efficiency of nitrogen utilization.