CAJ | 학술논문

蛋白免疫荧光(immunofluorescence, IF)分析是确定相关蛋白在染色体上具体位置的重要方法。本研究通过设计4种不同处理方式对水稻单倍体材料T2037的根尖染色体进行IF分析，发现4种处理方式对根尖染色体制备的效果不同。在没有酶解处理的条件下，经磷酸盐缓冲液(phosphate buffer solution, PBS)缓冲液保存的根尖比经过甲醇保存根尖的制片效果好，检测信号强；而酶解后的根尖染色体在两种不同处理方法下，染色体均有较重细胞质背景，检测信号弱。研究表明PBS保存材料且不进行酶解处理的根尖材料更适合于水稻染色体蛋白免疫分析。
단백면역형광(immunofluorescence, IF)분석시학정상관단백재염색체상구체위치적중요방법。본연구통과설계4충불동처리방식대수도단배체재료T2037적근첨염색체진행IF분석，발현4충처리방식대근첨염색체제비적효과불동。재몰유매해처리적조건하，경린산염완충액(phosphate buffer solution, PBS)완충액보존적근첨비경과갑순보존근첨적제편효과호，검측신호강；이매해후적근첨염색체재량충불동처리방법하，염색체균유교중세포질배경，검측신호약。연구표명PBS보존재료차불진행매해처리적근첨재료경괄합우수도염색체단백면역분석。
Protein Immunofluorescence (IF) analysis is an important method for determining related proteins on chromosome location. This work focused on influence on rice chromosome preparation by four different treatm-ents of root-tip in IF analysis. Without enzyme treatment, the result indicated the cytoplasm background of root-tips, preserved in PBS buffer, was weaker than that preserved in methanol. With enzyme treatment, chromosome morphology was poor in both preservation methods. The result showed that PBS to save material and no enzyme treatment were suitable for rice chromosome IF analysis.