分子植物育种
分子植物育種
분자식물육충
MOLECULAR PLANT BREEDING
2013年
6期
712-718
,共7页
何婷婷%建成%金寿林%朱高倩%徐莹洁%普世皇%谭学林
何婷婷%建成%金壽林%硃高倩%徐瑩潔%普世皇%譚學林
하정정%건성%금수림%주고천%서형길%보세황%담학림
籼粳亚种%恢复基因%Rf-1位点%PCR序列%变异
秈粳亞種%恢複基因%Rf-1位點%PCR序列%變異
선갱아충%회복기인%Rf-1위점%PCR서렬%변이
Indica-japonica subspecies%Fertility restorer gene%Rf-1 locus%PCR sequence%Variation
水稻育性恢复基因是鉴别籼粳亚种的关键基因。本研究利用育性恢复基因Rf-1位点上的特异性引物68923-8对来源不同的籼、粳稻恢复系及非恢复系进行PCR片段的序列比对及遗传距离分析。结果如下：在籼稻中，非恢复系间的一致性为94.97%，恢复系间的一致性为88.03%，恢复系与非恢复系之间的一致性达到91.56%；粳稻中，非恢复系间的一致性为91.56%，恢复系间一致性为57.48%，但恢复系与非恢复系间的一致性却较低，为36.53%；比较籼粳稻之间发现，二者非恢复系间的一致性为57.66%，恢复系间的一致性为54.71%。遗传距离的分析也揭示与此相似的结果。聚类将供试材料分成三大枝：第一大枝包含籼稻恢复系、籼稻非恢复系及1个粳稻恢复系Ansanbyeo；第二大枝包含6个粳稻非恢复系；而滇玉型恢复系南34与BT型恢复系C418则单独成第三大枝，而且与其它两枝的同源性较低。结果表明：水稻籼-粳亚种的遗传变异反映在育性恢复基因R f-1位点的DNA序列变异上。
水稻育性恢複基因是鑒彆秈粳亞種的關鍵基因。本研究利用育性恢複基因Rf-1位點上的特異性引物68923-8對來源不同的秈、粳稻恢複繫及非恢複繫進行PCR片段的序列比對及遺傳距離分析。結果如下：在秈稻中，非恢複繫間的一緻性為94.97%，恢複繫間的一緻性為88.03%，恢複繫與非恢複繫之間的一緻性達到91.56%；粳稻中，非恢複繫間的一緻性為91.56%，恢複繫間一緻性為57.48%，但恢複繫與非恢複繫間的一緻性卻較低，為36.53%；比較秈粳稻之間髮現，二者非恢複繫間的一緻性為57.66%，恢複繫間的一緻性為54.71%。遺傳距離的分析也揭示與此相似的結果。聚類將供試材料分成三大枝：第一大枝包含秈稻恢複繫、秈稻非恢複繫及1箇粳稻恢複繫Ansanbyeo；第二大枝包含6箇粳稻非恢複繫；而滇玉型恢複繫南34與BT型恢複繫C418則單獨成第三大枝，而且與其它兩枝的同源性較低。結果錶明：水稻秈-粳亞種的遺傳變異反映在育性恢複基因R f-1位點的DNA序列變異上。
수도육성회복기인시감별선갱아충적관건기인。본연구이용육성회복기인Rf-1위점상적특이성인물68923-8대래원불동적선、갱도회복계급비회복계진행PCR편단적서렬비대급유전거리분석。결과여하：재선도중，비회복계간적일치성위94.97%，회복계간적일치성위88.03%，회복계여비회복계지간적일치성체도91.56%；갱도중，비회복계간적일치성위91.56%，회복계간일치성위57.48%，단회복계여비회복계간적일치성각교저，위36.53%；비교선갱도지간발현，이자비회복계간적일치성위57.66%，회복계간적일치성위54.71%。유전거리적분석야게시여차상사적결과。취류장공시재료분성삼대지：제일대지포함선도회복계、선도비회복계급1개갱도회복계Ansanbyeo；제이대지포함6개갱도비회복계；이전옥형회복계남34여BT형회복계C418칙단독성제삼대지，이차여기타량지적동원성교저。결과표명：수도선-갱아충적유전변이반영재육성회복기인R f-1위점적DNA서렬변이상。
Fertility restorer gene (Rf) is a key gene to identify indica and japonica rice. In the present study, sequ-ences of PCR products by specific primer 68923-8 on Rf-1 locus of indica, japonica restorer and non-restorer lines in rice were analyzed. Results showed: in indica, genetic identity among indica non-restorers and restorers was 94.97%and 88.03%, respectively, and it was 91.56%between restorers and non-restorers;in japonica, The identity was 91.56%and 57.48%among japonica non-restorers and japonica restorers, respectively, and it was rather low, only 36.53%between non-restorers and restorers. The identity was 57.66%between indica and japonica lines of non-restorers, and it was 54.71%between indica-japonica lines of restorers. Analysis based on genetic distances gave the similar results. Cluster analysis showed all the materials divided into 3 main branches, the first main branch had 7 indica materials and 1 japonica restorer line from South Korea. The second main branch had 6 japonica non-restorers. And Nan34 and C418 got together in the third main branch, which had low identity with the other two branches. The results indicated that DNA sequences variation of Rf-1 locus reflected genetic differ-entiation of indica-japonica subspecies.
