CAJ | 학술논문

为研究共转化水稻的T-DNA共整合结构，本研究利用分别含有HPT、GFP和mCherry基因的T-DNA载体共转化水稻，从共转化植株获得了22个由两种T-DNA共整合形成的串联结构和25个关于相同T-DNA的多拷贝结构。序列分析结果表明，相邻T-DNA通过LRRL、RLLR和LRLR模式相互连接，由RB参与形成的T-DNA连接区均发生RB序列的完全缺失，在LB参与的T-DNA连接区，LB发生不同数目的碱基缺失或其3'末端的1个C碱基发生C→T颠换，因而使T-DNA连接区表现多种变异类型。此外，伴随着RB或LB边界的完全缺失，其边界内侧序列也缺失1~643 bp。在共转化水稻植株的T-DNA连接区不存在拟南芥相关报道所述的填充DNA序列。研究结果为深入解析禾谷类植物T-DNA共整合的分子机理奠定了重要基础。
위연구공전화수도적T-DNA공정합결구，본연구이용분별함유HPT、GFP화mCherry기인적T-DNA재체공전화수도，종공전화식주획득료22개유량충T-DNA공정합형성적천련결구화25개관우상동T-DNA적다고패결구。서렬분석결과표명，상린T-DNA통과LRRL、RLLR화LRLR모식상호련접，유RB삼여형성적T-DNA련접구균발생RB서렬적완전결실，재LB삼여적T-DNA련접구，LB발생불동수목적감기결실혹기3'말단적1개C감기발생C→T전환，인이사T-DNA련접구표현다충변이류형。차외，반수착RB혹LB변계적완전결실，기변계내측서렬야결실1~643 bp。재공전화수도식주적T-DNA련접구불존재의남개상관보도소술적전충DNA서렬。연구결과위심입해석화곡류식물T-DNA공정합적분자궤리전정료중요기출。
To investigate the co-integrated T-DNA structures in co-transformed rice plants, we co-transformed T-DNA vectors containing each of the HPT, GFP and mCherry genes into rice. Twenty-two tandem T-DNA struc-tures containing two T-DNA types and twenty-five multi-copy structures with one T-DNA type were identified in co-transformed rice plants. Based on the sequencing results, the co-integrated T-DNAs were linked into LRRL, RLLR and LRLR patterns. Deletion of complete RB sequence was observed in all the T-DNA junctions where RB was involved. In T-DNA junctions where LB was involved, the LB sequence lost various numbers of nucleotides or underwent a C→T transversion at the 3' end. For this reason, the LB-involved T-DNA junctions showed different types of sequence variation. Also, the deletion of whole LB or RB sequence was coincident with the loss of 1~643 nucleotides in LB or RB inward sequence. The T-DNA junctions in co-transformed rice plants did not contain any filler DNA that was previously identified in co-transformed Arabidopsis. These results provided important foundation for elucidation of the molecular mechanism of T-DNA co-integration in monocot plants.