分子植物育种
分子植物育種
분자식물육충
MOLECULAR PLANT BREEDING
2013年
6期
680-687
,共8页
闫成业%仲雪婷%Gandeka Mamadou%牟同敏
閆成業%仲雪婷%Gandeka Mamadou%牟同敏
염성업%중설정%Gandeka Mamadou%모동민
杂交水稻%Q优6号%白叶枯病抗性%分子标记辅助选择
雜交水稻%Q優6號%白葉枯病抗性%分子標記輔助選擇
잡교수도%Q우6호%백협고병항성%분자표기보조선택
Hybrid rice%Q-You 6%Bacterial blight resistance%MAS
杂交水稻组合Q优6号是长江中下游稻区大面积推广的中熟三系杂交中稻组合,对白叶枯病抗性较差。本研究采用杂交、回交和分子标记辅助选择技术,将广谱抗性基因X a23渗入到恢复系R1005中,综合农艺性状选择和X a23基因分子标记选择,获得1份具有X a23基因的优良株系CY11701-4。对该株系和以Q2A为母本配制的组合进行白叶枯病抗性鉴定,结果表明CY11701-4及Q2A/CY11701-4对接种的7个菌株(PXO61, PXO99, ZHE173, GD1358, FuJ, YN24和HeN11)均表现高抗水平。经主要农艺性状、产量和主要稻米品质指标测定分析,CY11701-4与R1005没有显著差异,Q2A/CY11701-4与Q优6号主要农艺性状没有显著差异,播始历期缩短1.3 d,产量显著提高。
雜交水稻組閤Q優6號是長江中下遊稻區大麵積推廣的中熟三繫雜交中稻組閤,對白葉枯病抗性較差。本研究採用雜交、迴交和分子標記輔助選擇技術,將廣譜抗性基因X a23滲入到恢複繫R1005中,綜閤農藝性狀選擇和X a23基因分子標記選擇,穫得1份具有X a23基因的優良株繫CY11701-4。對該株繫和以Q2A為母本配製的組閤進行白葉枯病抗性鑒定,結果錶明CY11701-4及Q2A/CY11701-4對接種的7箇菌株(PXO61, PXO99, ZHE173, GD1358, FuJ, YN24和HeN11)均錶現高抗水平。經主要農藝性狀、產量和主要稻米品質指標測定分析,CY11701-4與R1005沒有顯著差異,Q2A/CY11701-4與Q優6號主要農藝性狀沒有顯著差異,播始歷期縮短1.3 d,產量顯著提高。
잡교수도조합Q우6호시장강중하유도구대면적추엄적중숙삼계잡교중도조합,대백협고병항성교차。본연구채용잡교、회교화분자표기보조선택기술,장엄보항성기인X a23삼입도회복계R1005중,종합농예성상선택화X a23기인분자표기선택,획득1빈구유X a23기인적우량주계CY11701-4。대해주계화이Q2A위모본배제적조합진행백협고병항성감정,결과표명CY11701-4급Q2A/CY11701-4대접충적7개균주(PXO61, PXO99, ZHE173, GD1358, FuJ, YN24화HeN11)균표현고항수평。경주요농예성상、산량화주요도미품질지표측정분석,CY11701-4여R1005몰유현저차이,Q2A/CY11701-4여Q우6호주요농예성상몰유현저차이,파시력기축단1.3 d,산량현저제고。
Q-You 6, a three-line hybrid rice variety planting in Yangtze River Basin, is susceptible to bacterial blight caused the yield losses. We introgressed a bacterial blight resistance gene Xa23 from JYQ9008 into the male parent R1005 of Q-You 6 by molecular marker assisted selection and phenotypic selection. One line, CY11701-4, carrying X a23 gene under the R1005 background was developed and crossed with Q2A, the female parent of Q-You 6. The results showed that CY11701-4 and Q2A/CY11701-4 were resistant to seven Xoo strains, including PXO61, PXO99, ZHE173, GD1358, FuJ, YN24 and HeN11. The evaluation results of grain quality, yield and yield-related traits showed that CY11701-4 and Q2A/CY11701-4 were as similar to R1005 and Q-You 6, res-pectively.