CAJ | 학술논문

采用高效液相色谱-电喷雾串联质谱仪（LC -ESI-MS-MS），建立猪肉中林可霉素、竹桃霉素、替米考星、泰乐菌素、克林霉素、吉他霉素、交沙霉素等7种大环内酯类药物残留的检测方法，试验中猪肉中大环内酯类抗生素的残留用乙腈提取，以罗红霉素作为内标，提取液用正己烷去除脂肪后浓缩，再用磷酸盐溶液溶解后，经HLB固相萃取柱净化，甲醇洗脱，洗脱液浓缩定容至1mL ，供液相色谱-串联质谱法测定，内标法定量。本方法采用10μg/kg ，20μg/kg ，100μg/kg ，3个添加浓度，每个浓度6个平行样品，上述7种大环内酯类药物残留的回收率在75％～100％，相对偏差在1.51％～5.48％。
채용고효액상색보-전분무천련질보의（LC -ESI-MS-MS），건립저육중림가매소、죽도매소、체미고성、태악균소、극림매소、길타매소、교사매소등7충대배내지류약물잔류적검측방법，시험중저육중대배내지류항생소적잔류용을정제취，이라홍매소작위내표，제취액용정기완거제지방후농축，재용린산염용액용해후，경HLB고상췌취주정화，갑순세탈，세탈액농축정용지1mL ，공액상색보-천련질보법측정，내표법정량。본방법채용10μg/kg ，20μg/kg ，100μg/kg ，3개첨가농도，매개농도6개평행양품，상술7충대배내지류약물잔류적회수솔재75％～100％，상대편차재1.51％～5.48％。
A method was established for the determination of seven kinds of macrolide drug residue in pork ,seven kinds of macrolide drug were lincomycin ,oleandomycin ,tilmicosin ,tylosin ,clindamycin , kitasamycin ,josamycin. The sample was extracted with acetonitrile. With roxithromycin as an internal standard ,extract was washed with n-hexane to remove fat and then concentrated ,phosphate solution dis-solved ,cleaned by the HLB solid phase extraction (SPE) ,methanol eluate and concentrated to volume 1mL ,for liquid chromatography-tandem mass spectrometry determination ,internal standard. This method uses 10μg/kg ,20μg/kg ,100μg/kg ,3 spiked concentration ,each concentration six parallel samples .The seven kinds of macrolide drug residues recovery is 75%-100% ,the relative deviation is 1.51%-5.48%.