CAJ | 학술논문

建立巴鱼中呋喃唑酮、呋喃它酮、呋喃西林和呋喃妥因的代谢物残留量液相色谱-串联质谱的方法。分析物采用电喷雾电离正离子（ ESI ＋）、多反应监测（M RM ）模式检测，内标法定量。本实验对固相萃取法提取和乙酸乙酯提取净化前处理方法进行比对，4种硝基呋喃代谢物的混合标准溶液的浓度在0.5～50ng/mL范围线性良好，相关系数均大于0.9981，4种化合物的检测限为0.5μg/kg。添加浓度1μg/kg、2.5μg/kg、5μg/kg范围内得出4种代谢物固相萃取净化法的回收率在81.1％～104.1％之间，相对标准偏差（RSD）小于5.7％.乙酸乙酯提取净化法的回收率在88.5％～108.2％之间，相对标准偏差（RSD ）小于6.1％。结论证明乙酸乙酯提取法方法前处理简单、快速、稳定性好，适用于大量样品的同时检测。
건립파어중부남서동、부남타동、부남서림화부남타인적대사물잔류량액상색보-천련질보적방법。분석물채용전분무전리정리자（ ESI ＋）、다반응감측（M RM ）모식검측，내표법정량。본실험대고상췌취법제취화을산을지제취정화전처리방법진행비대，4충초기부남대사물적혼합표준용액적농도재0.5～50ng/mL범위선성량호，상관계수균대우0.9981，4충화합물적검측한위0.5μg/kg。첨가농도1μg/kg、2.5μg/kg、5μg/kg범위내득출4충대사물고상췌취정화법적회수솔재81.1％～104.1％지간，상대표준편차（RSD）소우5.7％.을산을지제취정화법적회수솔재88.5％～108.2％지간，상대표준편차（RSD ）소우6.1％。결론증명을산을지제취법방법전처리간단、쾌속、은정성호，괄용우대량양품적동시검측。
A method was established to detect nitrofuran metabolic residues in Spanish mackerel by liq-uid chromatography tandem mass spetrometry (LC-MS/MS) .Theanalvtes were detected by LC-MS/MS (ESI) with multiple reaction monitoring (MRM ).The result indicated that the calibration curves were in good linearity between the peak area and the concentration of 0.5-50ng/ml for nitrofuran metabolic with correlation cofficients not less than 0.9981.The limits of detection for four nitrofuran metabolics were 0.5μg/kg. .Then analytes were extracted by spe or ethyl acetat the average recoveries were 81.1%-104.1% or 88.5%-108.2% and the related standard deviations less than 5.1 or 6.1% at the three levels 1μg/kg、2.5μg/kg、5μg/kg.The proposed method was simple ,rapid ,the stability was good and suitable for the simultaneous quantitative detection.