CAJ | 학술논문

目的：建立HPLC测定广西不同产地龙眼花药材中槲皮素、木犀草素和山奈素含量的方法。方法：Hypersil C18色谱柱（250 mm×4.6 mm，5μm），流动相为甲醇-0.2%磷酸（47：53），流速1.0 mL·min-1，柱温30℃，检测波长360 nm。结果：槲皮素、木犀草素和山柰素分别在0.18~2.88μg、0.059~0.944μg和0.024~0.384μg之间呈良好的线性关系（r=0.9999），平均加样回收率分别为100.06%、99.77%、98.67%， RSD分别为1.72%、1.18%、1.99%（n=9）。结论：该方法简便可靠，快速，重复性好，可用于龙眼花药材的质量控制。
목적：건립HPLC측정엄서불동산지용안화약재중곡피소、목서초소화산내소함량적방법。방법：Hypersil C18색보주（250 mm×4.6 mm，5μm），류동상위갑순-0.2%린산（47：53），류속1.0 mL·min-1，주온30℃，검측파장360 nm。결과：곡피소、목서초소화산내소분별재0.18~2.88μg、0.059~0.944μg화0.024~0.384μg지간정량호적선성관계（r=0.9999），평균가양회수솔분별위100.06%、99.77%、98.67%， RSD분별위1.72%、1.18%、1.99%（n=9）。결론：해방법간편가고，쾌속，중복성호，가용우용안화약재적질량공제。
This study was aimed to create a method for content determination of quercetin, luteolin and kaempferol in flowers of Dimocarpus longan Lour. from different habitats of Guangxi province by HPLC. The samples were sepa-rated on the Hypersil C18 column (250 mm í 4.6 mm, 5 μm) which was eluted with methanol-0.2% phosphoric acid solution (47:53) with detective wavelength at 360 nm, flow rate at 1.0 mL·min-1, and column temperature at 30℃. The results showed that this method had a good linear relationship within the range of 0.18 to 2.88 μg for quercetin, 0.059 to 0.944 μg for luteolin, and 0.024 to 0.384 μg for kaempferol (r = 0.9999). The average recovery rate was 100.06% (RSD = 1.72%), 99.77% (RSD = 1.18%) and 98.67% (RSD = 1.99%, n = 9), respectively. It was conclud-ed that this method is simple, rapid, reliable and with good repeatability, which can be used in the quality control of flowers of Dimocarpus longan Lour.