世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2013年
7期
1562-1568
,共7页
孟盼%王宇红%张秀丽%蔡川%韩远山
孟盼%王宇紅%張秀麗%蔡川%韓遠山
맹반%왕우홍%장수려%채천%한원산
百事乐胶囊%抑郁症%海马%突触素玉%突触囊泡素
百事樂膠囊%抑鬱癥%海馬%突觸素玉%突觸囊泡素
백사악효낭%억욱증%해마%돌촉소옥%돌촉낭포소
Baishile Capsule%depression%hippocampus%SYN I%SYNA
目的：探讨百事乐胶囊对慢性应激抑郁模型大鼠行为活动、学习记忆及海马CA3区突触素玉（SYN玉）和突触囊泡素（SYNA）表达的影响。方法：SD大鼠随机分为6组，即空白对照组、抑郁模型组、氟西汀组、百事乐胶囊（2.88 g·kg-1、1.44 g·kg-1、0.72 g·kg-1）组，采用慢性温和不可预见性应激加孤养的方式建立抑郁模型，造模同时灌胃给药，每日1次，连续给药21天，对照组、模型组等量蒸馏水灌胃，观察大鼠Open-field、1%蔗糖偏食度、水迷宫及体质量变化率的影响，免疫组化和原位杂交观察各组大鼠SYN玉和SYNA的表达变化。结果：行为学表明，百事乐高剂量治疗2周后可显著提升模型大鼠水平及垂直活动次数（P<0.01），百事乐中剂量治疗3周后可明显提升模型大鼠水平活动次数（P<0.05）。高剂量治疗第1周后、中剂量治疗3周后可明显提高模型大鼠的蔗糖偏食度（P<0.01或P<0.05）。高剂量治疗2周、中剂量治疗3周可明显提高模型大鼠的体质量变化率（P<0.01或P<0.05）。百事乐高、中剂量可以缩短模型大鼠的寻找并爬上平台的持续时间（EL），并能明显升高模型大鼠穿越目标象限的次数；同时高剂量可明显缩短目标象限潜伏时间（P<0.05）。免疫组化和原位杂交结果显示，与模型组比较，百事乐高剂量能明显促进模型大鼠海马CA3区SYN玉和SYNA的表达，中剂量可明显提升SYNA的表达（P<0.05或P<0.01）。结论：百事乐胶囊能改善抑郁模型大鼠的抑郁行为及海马CA3区SYN玉和SYNA的表达。
目的：探討百事樂膠囊對慢性應激抑鬱模型大鼠行為活動、學習記憶及海馬CA3區突觸素玉（SYN玉）和突觸囊泡素（SYNA）錶達的影響。方法：SD大鼠隨機分為6組，即空白對照組、抑鬱模型組、氟西汀組、百事樂膠囊（2.88 g·kg-1、1.44 g·kg-1、0.72 g·kg-1）組，採用慢性溫和不可預見性應激加孤養的方式建立抑鬱模型，造模同時灌胃給藥，每日1次，連續給藥21天，對照組、模型組等量蒸餾水灌胃，觀察大鼠Open-field、1%蔗糖偏食度、水迷宮及體質量變化率的影響，免疫組化和原位雜交觀察各組大鼠SYN玉和SYNA的錶達變化。結果：行為學錶明，百事樂高劑量治療2週後可顯著提升模型大鼠水平及垂直活動次數（P<0.01），百事樂中劑量治療3週後可明顯提升模型大鼠水平活動次數（P<0.05）。高劑量治療第1週後、中劑量治療3週後可明顯提高模型大鼠的蔗糖偏食度（P<0.01或P<0.05）。高劑量治療2週、中劑量治療3週可明顯提高模型大鼠的體質量變化率（P<0.01或P<0.05）。百事樂高、中劑量可以縮短模型大鼠的尋找併爬上平檯的持續時間（EL），併能明顯升高模型大鼠穿越目標象限的次數；同時高劑量可明顯縮短目標象限潛伏時間（P<0.05）。免疫組化和原位雜交結果顯示，與模型組比較，百事樂高劑量能明顯促進模型大鼠海馬CA3區SYN玉和SYNA的錶達，中劑量可明顯提升SYNA的錶達（P<0.05或P<0.01）。結論：百事樂膠囊能改善抑鬱模型大鼠的抑鬱行為及海馬CA3區SYN玉和SYNA的錶達。
목적：탐토백사악효낭대만성응격억욱모형대서행위활동、학습기억급해마CA3구돌촉소옥（SYN옥）화돌촉낭포소（SYNA）표체적영향。방법：SD대서수궤분위6조，즉공백대조조、억욱모형조、불서정조、백사악효낭（2.88 g·kg-1、1.44 g·kg-1、0.72 g·kg-1）조，채용만성온화불가예견성응격가고양적방식건립억욱모형，조모동시관위급약，매일1차，련속급약21천，대조조、모형조등량증류수관위，관찰대서Open-field、1%자당편식도、수미궁급체질량변화솔적영향，면역조화화원위잡교관찰각조대서SYN옥화SYNA적표체변화。결과：행위학표명，백사악고제량치료2주후가현저제승모형대서수평급수직활동차수（P<0.01），백사악중제량치료3주후가명현제승모형대서수평활동차수（P<0.05）。고제량치료제1주후、중제량치료3주후가명현제고모형대서적자당편식도（P<0.01혹P<0.05）。고제량치료2주、중제량치료3주가명현제고모형대서적체질량변화솔（P<0.01혹P<0.05）。백사악고、중제량가이축단모형대서적심조병파상평태적지속시간（EL），병능명현승고모형대서천월목표상한적차수；동시고제량가명현축단목표상한잠복시간（P<0.05）。면역조화화원위잡교결과현시，여모형조비교，백사악고제량능명현촉진모형대서해마CA3구SYN옥화SYNA적표체，중제량가명현제승SYNA적표체（P<0.05혹P<0.01）。결론：백사악효낭능개선억욱모형대서적억욱행위급해마CA3구SYN옥화SYNA적표체。
This study was aimed to investigate Baishile Capsule on behavior, memory and expression of SYN I and SYNA in hippocampal CA3 region of depression model rats. SD rats were randomly divided into six groups, which were the control group, depression model group, fluoxetine hydrochloride group, and the Baishile Capsule group (2.88 g·kg-1, 1.44 g·kg-1, 0.72 g·kg-1). The chronic stress depression model rats were established by chronic and mild unpredictable stressors as described in the literature. In the model group, medicine was intragastricly administered once per day and continued for 21 days. In the control group and model group, same volume of distilled water was intragastricly administered. The open-field test, preference for 1% sucrose solution, Morris water maze, and rate of weight were carried out and observed. Immunohistochemistry and in situ hybridization were used in the observation of the expression of SYN I and SYNA in each group of model rats. The results showed that high-dosage Baishile Capsule can significantly increase the horizontal and vertical activities of score after two-week treatment (P< 0.01). The middle-dosage Baishile Capsule can significantly increase the horizontal activity of score after three-week treatment (P< 0.05). The rat's preference for sucrose solution was obviously increased in the high-dosage group after one-week treatment and in the middle-dosage group after three-week treatment (P< 0.01, or P< 0.05). The rate of weight of rats was obviously increased in the high-dosage group after two-week treatment and in the middle-dosage group after three-week treatment (P< 0.01, or P< 0.05). The high-dosage and middle-dosage Baishile Capsule can shorten EL, and significantly increase the number of target quadrant. The high-dosage Baishile Capsule can obviously shorten the target quadrant latency (P< 0.05). Compared with the model group, the immunohistochemistry and in situ hybridization showed high-dosage Baishile can significantly promote the expression of SYN I and SYNA in hippocampal CA3 region; and the middle-dosage group can enhance the expression of SYNA (P < 0.05, or P <0.01). It was concluded that Baishile Capsule can obviously improve the behavior and the expression of SYN I and SYNA in hippocampal CA3 region of depression model rats.
