茶叶科学
茶葉科學
다협과학
2013年
6期
590-597
,共8页
水培茶树%铅累积%TEM-EDS%超微定位
水培茶樹%鉛纍積%TEM-EDS%超微定位
수배다수%연루적%TEM-EDS%초미정위
hydroponic tea%Pb accumulation%TEM-EDS%ultra-structure localization
应用水培茶树模式,采用透射电子显微镜(Transmission Electron Microscope,TEM)和X射线能谱仪(Energy Dispersive Spectrometer,EDS)相结合的手段,对铅盐水培茶树根、茎、叶细胞内Pb2+的累积部位与分布水平进行观测与分析。结果表明:茶树根细胞中的铅均匀地分布于细胞壁上,且数量最多,质膜、细胞质和液泡膜上的铅较少,细胞间隙等部位的铅最少;嫩茎细胞壁中的铅颗粒小、数量多,质膜、液泡膜及叶绿体上数量较少;叶片细胞中的铅则主要分布在质膜和液泡膜上,细胞壁中的铅较少。
應用水培茶樹模式,採用透射電子顯微鏡(Transmission Electron Microscope,TEM)和X射線能譜儀(Energy Dispersive Spectrometer,EDS)相結閤的手段,對鉛鹽水培茶樹根、莖、葉細胞內Pb2+的纍積部位與分佈水平進行觀測與分析。結果錶明:茶樹根細胞中的鉛均勻地分佈于細胞壁上,且數量最多,質膜、細胞質和液泡膜上的鉛較少,細胞間隙等部位的鉛最少;嫩莖細胞壁中的鉛顆粒小、數量多,質膜、液泡膜及葉綠體上數量較少;葉片細胞中的鉛則主要分佈在質膜和液泡膜上,細胞壁中的鉛較少。
응용수배다수모식,채용투사전자현미경(Transmission Electron Microscope,TEM)화X사선능보의(Energy Dispersive Spectrometer,EDS)상결합적수단,대연염수배다수근、경、협세포내Pb2+적루적부위여분포수평진행관측여분석。결과표명:다수근세포중적연균균지분포우세포벽상,차수량최다,질막、세포질화액포막상적연교소,세포간극등부위적연최소;눈경세포벽중적연과립소、수량다,질막、액포막급협록체상수량교소;협편세포중적연칙주요분포재질막화액포막상,세포벽중적연교소。
This study applied lead salts hydroponic tea plant, observed and analyzed Pb accumulation and ultra-structure localization in the cells of root, stem, leaves by the TEM-EDS method. The results showed that most of Pb evenly distributed on the cell wall of root cells, only few Pb distributed on the plasma, cytoplasm, vacuole membrane and intercellular space. While, Pb on cell wall of stem were numerals and small, and a little on plasma, vacuole membrane and chloroplast. Pb in leaves were mainly on plasma and vacuole membrane, there were only a little on the cell wall of leaves.