CAJ | 학술논문

茶树叶片中含有丰富的代谢产物，氨基酸、茶多酚和咖啡碱是决定茶叶品质的重要成分。本文结合基于1H-核磁共振（1H-NMR）的代谢组学非靶标分析与通过高效液相色谱法进行的定量靶标分析两种方法，比较白天和夜晚所取不同施氮水平的茶树新梢第2叶的代谢产物差异，探讨了基于1H-NMR 的代谢组学研究方法应用于茶叶品质成分形成机理研究的可行性。结果表明，白天和夜晚所取茶树新梢第2叶代谢组差异较大，可以显著分离，区分两个样本的主要组分为茶氨酸、葡萄糖和蔗糖，而不同施氮水平下茶树新梢第2叶代谢组没有显著差异。高效液相色谱定量分析的结果表明，施氮水平对主要游离氨基酸组分和儿茶素组分均有影响，且存在昼夜差别。
다수협편중함유봉부적대사산물，안기산、다다분화가배감시결정다협품질적중요성분。본문결합기우1H-핵자공진（1H-NMR）적대사조학비파표분석여통과고효액상색보법진행적정량파표분석량충방법，비교백천화야만소취불동시담수평적다수신소제2협적대사산물차이，탐토료기우1H-NMR 적대사조학연구방법응용우다협품질성분형성궤리연구적가행성。결과표명，백천화야만소취다수신소제2협대사조차이교대，가이현저분리，구분량개양본적주요조분위다안산、포도당화자당，이불동시담수평하다수신소제2협대사조몰유현저차이。고효액상색보정량분석적결과표명，시담수평대주요유리안기산조분화인다소조분균유영향，차존재주야차별。
Amino acids, polyphenols, caffeine and other metabolites such as sugars and aromatic substances are important determinants of tea quality. Different cultivation practices affect tea quality by altering the basic metabolite profiles. In this study, the metabolomic changes through nitrogen and samples collected in day and night were investigated in fresh tea shoots by 1H-NMR for non-targeted analysis coupled with a multivariate data analysis and by HPLC for targeted analysis to reveal the mechanism of formation, transformation and relationship of the major characteristic quality components in fresh tea leaves. Non-targeted analysis with 1H-NMR showed significant effect of samples collected in day and night but no difference among N application rates. However, targeted analysis with HPLC showed both significant variations in amino acids and catechins as affected by N rates and light/dark change. There were higher sensitivity of amino acids and sugars but lower sensitivity of tea polyphenols through the metabolomic analysis using 1H-NMR.