CAJ | 학술논문

细胞粘附与铺展是三维水凝胶基质中贴壁依赖型细胞存活所必须的两个条件，将细胞粘附位点的引入和凝胶中细胞铺展空间的构建相结合，提出了同时含有RGD多肽和明胶微球的粘附型大孔水凝胶模型，以促进细胞在其中的铺展与分化。该模型采用光交联海藻酸钠水凝胶为基础，同时引入RGD多肽和明胶微球，通过RGD多肽的共价接枝为细胞粘附提供前提，利用明胶微球在37℃下的快速降解性，为细胞的进一步增殖和铺展以及分化提供所需空间。结果显示，明胶微球的加入提高了凝胶的力学性能，同时降低了凝胶的溶胀率。 RGD和明胶微球的引入能够很好地支持 MG-63细胞在其中的增殖、粘附与铺展，并显著提高其碱性磷酸酶活性，上调成骨相关基因（ BMP-2， COL-I和 OCN）的表达。而在不含微球的 RGD-ALG 和 ALG凝胶中，细胞铺展及成骨分化均受到很大抑制。
세포점부여포전시삼유수응효기질중첩벽의뢰형세포존활소필수적량개조건，장세포점부위점적인입화응효중세포포전공간적구건상결합，제출료동시함유RGD다태화명효미구적점부형대공수응효모형，이촉진세포재기중적포전여분화。해모형채용광교련해조산납수응효위기출，동시인입RGD다태화명효미구，통과RGD다태적공개접지위세포점부제공전제，이용명효미구재37℃하적쾌속강해성，위세포적진일보증식화포전이급분화제공소수공간。결과현시，명효미구적가입제고료응효적역학성능，동시강저료응효적용창솔。 RGD화명효미구적인입능구흔호지지지 MG-63세포재기중적증식、점부여포전，병현저제고기감성린산매활성，상조성골상관기인（ BMP-2， COL-I화 OCN）적표체。이재불함미구적 RGD-ALG 화 ALG응효중，세포포전급성골분화균수도흔대억제。
Adhesion and spreading of anchorage-dependent cells are two essential factors for their subsequent settlement and commitment in 3D hydrogel substrate.An adhesive macroporous alginate hydrogel system was suggested here to over-come the low cell affinity and high cell constraint of hydrogel substrates for cells encapsulated in them .RGD peptide was grafted onto the alginate molecule to support cell adhesion and gelatin microspheres were introduced into photocrosslinked alginate hydrogels to create macropores by their degradation at 37℃for cell spreading and facilitate cell ingrowth as well as prolonged cell survival within hydrogels .With the incorporation of gelatin microspheres , the hybrid alginate hydrogels ex-hibited significantly enhanced mechanical properties and decreased swelling ratios .MG-63 cells in alginate hydrogel coop-erated with RGD and gelatin microspheres exhibited a rapid proliferation and spread gradually with prolonged culture time , and their osteogenic differentiation was greatly facilitated, such as the high expression of BMP-2, COL-I and OCN genes. While in the pure alginate hydrogel ( ALG) and hydrogels only capable of cell affinity ( RGD-ALG) , cells could just ex-hibit either a rounded shape or a spreading morphology with a very limited degree , and the osteogenic differentiation was also inhibited.