中国材料进展
中國材料進展
중국재료진전
MATERIALS CHINA
2013年
10期
605-610
,共6页
磁性纳米颗粒%基因递送%磁转染%载体
磁性納米顆粒%基因遞送%磁轉染%載體
자성납미과립%기인체송%자전염%재체
magnetic nanoparticles%gene delivery%magnetofection%carrier
基因递送是实现基因治疗的关键，基因的有效递送有赖于发展安全有效的递送载体。理想的基因递送载体应具备递送效率高、细胞毒性低、对正常细胞生理影响小以及易于使用和重复等特性。纳米材料独特的理化性质使其在药物和基因递送领域具有潜在的应用，尤其是磁性纳米颗粒，其兼具纳米效应和超顺磁性，是一种非常有应用前景的载体材料。然而磁性纳米颗粒的基因递送效率受多种因素的影响，包括纳米颗粒的类型、粒径、表面特性和外加磁场等。因此，为了能够进行有效基因递送，应综合考虑设计磁性纳米颗粒。目前，磁性纳米颗粒已经成功应用于基因的体外转染，成为细胞生物学研究的重要工具，然而，其在体内基因递送的应用方面还存在着诸多问题，有待进一步深入研究。
基因遞送是實現基因治療的關鍵，基因的有效遞送有賴于髮展安全有效的遞送載體。理想的基因遞送載體應具備遞送效率高、細胞毒性低、對正常細胞生理影響小以及易于使用和重複等特性。納米材料獨特的理化性質使其在藥物和基因遞送領域具有潛在的應用，尤其是磁性納米顆粒，其兼具納米效應和超順磁性，是一種非常有應用前景的載體材料。然而磁性納米顆粒的基因遞送效率受多種因素的影響，包括納米顆粒的類型、粒徑、錶麵特性和外加磁場等。因此，為瞭能夠進行有效基因遞送，應綜閤攷慮設計磁性納米顆粒。目前，磁性納米顆粒已經成功應用于基因的體外轉染，成為細胞生物學研究的重要工具，然而，其在體內基因遞送的應用方麵還存在著諸多問題，有待進一步深入研究。
기인체송시실현기인치료적관건，기인적유효체송유뢰우발전안전유효적체송재체。이상적기인체송재체응구비체송효솔고、세포독성저、대정상세포생리영향소이급역우사용화중복등특성。납미재료독특적이화성질사기재약물화기인체송영역구유잠재적응용，우기시자성납미과립，기겸구납미효응화초순자성，시일충비상유응용전경적재체재료。연이자성납미과립적기인체송효솔수다충인소적영향，포괄납미과립적류형、립경、표면특성화외가자장등。인차，위료능구진행유효기인체송，응종합고필설계자성납미과립。목전，자성납미과립이경성공응용우기인적체외전염，성위세포생물학연구적중요공구，연이，기재체내기인체송적응용방면환존재착제다문제，유대진일보심입연구。
Effective gene delivery is critical for gene therapy and depends on the development of safe and effective deliv-ery carrier.Ideal gene delivery carrier should possess plenty of good properties, including high delivery efficiency, low cy-totoxicity, less effect on physiological function of normal cells, and ease of use and duplication.Nanomaterials have poten-tial applications in the field of drug and gene delivery due to their unique physical and chemical properties .Especially, magnetic nanoparticles ( MNPs) with nano-effect and superparamagnetic properties are very promising carrier for gene de-livery.However, gene delivery efficiency of MNPs is affected by multiple factors, including the types, sizes and surface properties of nanoparticles and external magnetic field , which should be considered synthetically for designing MNPs so as to achieve higher gene delivery efficiency.Currently, MNPs have been successfully used for in vitro gene transfection as a powerful tool in cellular and molecular biology.However, many problems about in vivo gene therapy of MNPs still exist, which remain to be further studied .
