CAJ | 학술논문

微纳米生物材料目前已成为生物医用材料领域一个研究热点和难点。大量研究表明具有微纳米结构特征的生物材料表现出了积极的生物学响应。生物活性玻璃（ BG）具有较高的生物活性、生物相容性，是一类重要的骨修复材料。而微纳米生物活性玻璃（ MNBG）因其具有特殊的形态结构和理化性能，引起众多研究者的关注。但是目前对 MNBG的研究还主要集中在制备、表征以及其表面类骨羟基磷灰石矿物在SBF溶液中的形成活性等方面，关于MNBG的细胞相容性以及基因激活性能方面的研究还鲜有报道。通过溶胶-凝胶法结合模板仿生技术合成了具有特殊微纳米结构和形态的MNBG，并将其浸提液与MG-63细胞共培养，研究生物玻璃溶出物对细胞增殖，成骨相关基因和蛋白表达的影响，结果证明相比于传统的熔融法制备的生物玻璃（45S5）浸提液， MNBG浸提液能够明显促进细胞增殖，激活细胞成骨相关基因，上调相关蛋白的表达，为设计和制备具有基因介导作用的新型生物活性玻璃骨修复材料提供了理论依据。
미납미생물재료목전이성위생물의용재료영역일개연구열점화난점。대량연구표명구유미납미결구특정적생물재료표현출료적겁적생물학향응。생물활성파리（ BG）구유교고적생물활성、생물상용성，시일류중요적골수복재료。이미납미생물활성파리（ MNBG）인기구유특수적형태결구화이화성능，인기음다연구자적관주。단시목전대 MNBG적연구환주요집중재제비、표정이급기표면류골간기린회석광물재SBF용액중적형성활성등방면，관우MNBG적세포상용성이급기인격활성능방면적연구환선유보도。통과용효-응효법결합모판방생기술합성료구유특수미납미결구화형태적MNBG，병장기침제액여MG-63세포공배양，연구생물파리용출물대세포증식，성골상관기인화단백표체적영향，결과증명상비우전통적용융법제비적생물파리（45S5）침제액， MNBG침제액능구명현촉진세포증식，격활세포성골상관기인，상조상관단백적표체，위설계화제비구유기인개도작용적신형생물활성파리골수복재료제공료이론의거。
Micro/nano biological material has become the research hotspot and difficulty in the field of biomedical mate-rials.A number of studies have shown that micro/nano structure of biological material can show a positive biological re-sponse.Bioactive glass ( BG) has the high apatite-forming bioactivity and biocompatibility.BG is the ideal biomaterial for bone tissue regeneration.Micro/nano-bioactive glass ( MNBG) caused much attention because of the special morphology and physicochemical properties, but the research about MNBG only focused on preparation and the in vitro apatite-forming bioactivity, few papers have focused on the biocompatibility and gene activation .This study used sol-gel method combining biological organic templates to design micro/nano structure of bioactive glasses.The effects of MNBG and traditional melt 45S5 ionic dissolution products on proliferation, expression of osteogenic genes, proteins were investigated.The results showed that MNBG promoted cell-proliferation, up-regulated the expression of osteogenic genes and proteins.This work will provide a reference for design of specific morphology of the bioactive bone repair materials in the future .