中国血液流变学杂志
中國血液流變學雜誌
중국혈액류변학잡지
CHINESE JOURNAL OF HEMORHEOLOGY
2013年
3期
498-500
,共3页
人乳头瘤病毒%宫颈病变%基因分型
人乳頭瘤病毒%宮頸病變%基因分型
인유두류병독%궁경병변%기인분형
human papilloma virus%cervix disease%genotyping
目的:探讨妇科门诊病人不同程度的子宫颈病变感染人乳头瘤病毒(human papilloma virus,HPV)的基因亚型,以探讨不同基因型HPV感染的致癌性。方法对160例有宫颈糜烂Ⅰ-Ⅲ度的妇科门诊患者应用聚合酶链反应(Polymerase Chain Reaction,PCR)方法复制扩增病原DNA,再利用膜杂交技术进行基因分型检测HPV亚型,同时对宫颈脱落细胞进行膜式液基超薄细胞学检测(Thinprep cytologic test,TCT),或在阴道镜下行宫颈活检、经病理学诊断确诊。根据细胞学与病理学诊断有无宫颈病变及其病变程度分5组。结果(1)HPV的感染率:宫颈慢性炎为17.3%(13/75),慢性炎见挖空细胞为70.4%(19/27),低度鳞状上皮内瘤变CINⅠ为23.1%(3/13),高度鳞状上皮内瘤变(CINⅡ-Ⅲ)为55.6%(20/36),宫颈癌为88.9%(8/9)。(2)分析HPV感染基因型与宫颈病变程度的关系,CINⅡ-Ⅲ患者中单一高危型HPV感染率60.0%,明显高于混合型(35.0%)和单一低危型(5.0%),其感染的HPV型别有16、18、31、33、45、56、58、73,其中16型感染率为75.0%,具有统计学意义。结论 HPV高危型与宫颈病变程度密切相关,随着宫颈病变程度加重,HPV高度型感染有增加趋势。
目的:探討婦科門診病人不同程度的子宮頸病變感染人乳頭瘤病毒(human papilloma virus,HPV)的基因亞型,以探討不同基因型HPV感染的緻癌性。方法對160例有宮頸糜爛Ⅰ-Ⅲ度的婦科門診患者應用聚閤酶鏈反應(Polymerase Chain Reaction,PCR)方法複製擴增病原DNA,再利用膜雜交技術進行基因分型檢測HPV亞型,同時對宮頸脫落細胞進行膜式液基超薄細胞學檢測(Thinprep cytologic test,TCT),或在陰道鏡下行宮頸活檢、經病理學診斷確診。根據細胞學與病理學診斷有無宮頸病變及其病變程度分5組。結果(1)HPV的感染率:宮頸慢性炎為17.3%(13/75),慢性炎見挖空細胞為70.4%(19/27),低度鱗狀上皮內瘤變CINⅠ為23.1%(3/13),高度鱗狀上皮內瘤變(CINⅡ-Ⅲ)為55.6%(20/36),宮頸癌為88.9%(8/9)。(2)分析HPV感染基因型與宮頸病變程度的關繫,CINⅡ-Ⅲ患者中單一高危型HPV感染率60.0%,明顯高于混閤型(35.0%)和單一低危型(5.0%),其感染的HPV型彆有16、18、31、33、45、56、58、73,其中16型感染率為75.0%,具有統計學意義。結論 HPV高危型與宮頸病變程度密切相關,隨著宮頸病變程度加重,HPV高度型感染有增加趨勢。
목적:탐토부과문진병인불동정도적자궁경병변감염인유두류병독(human papilloma virus,HPV)적기인아형,이탐토불동기인형HPV감염적치암성。방법대160례유궁경미란Ⅰ-Ⅲ도적부과문진환자응용취합매련반응(Polymerase Chain Reaction,PCR)방법복제확증병원DNA,재이용막잡교기술진행기인분형검측HPV아형,동시대궁경탈락세포진행막식액기초박세포학검측(Thinprep cytologic test,TCT),혹재음도경하행궁경활검、경병이학진단학진。근거세포학여병이학진단유무궁경병변급기병변정도분5조。결과(1)HPV적감염솔:궁경만성염위17.3%(13/75),만성염견알공세포위70.4%(19/27),저도린상상피내류변CINⅠ위23.1%(3/13),고도린상상피내류변(CINⅡ-Ⅲ)위55.6%(20/36),궁경암위88.9%(8/9)。(2)분석HPV감염기인형여궁경병변정도적관계,CINⅡ-Ⅲ환자중단일고위형HPV감염솔60.0%,명현고우혼합형(35.0%)화단일저위형(5.0%),기감염적HPV형별유16、18、31、33、45、56、58、73,기중16형감염솔위75.0%,구유통계학의의。결론 HPV고위형여궁경병변정도밀절상관,수착궁경병변정도가중,HPV고도형감염유증가추세。
Objective To investigate human papilloma virus types detected in outpatients with different degree diseases at cervix and to analyze the carcinogenicity of the various human papilloma virus(HPV) types.Methods 160 outpatients of gynecology who have different degree cervical erosion were tested with polymerase chain reaction to duplicate etiological agent’s DNA and membrane hybridization technique to Genotyping detect HPV hypotype.At the same time Thinprep cytologic test(TCT) were used to detect their cast-off cell or under colposcope gynecatoptron to cervical biopsy pathology to diagnose it.According to the cytological and pathological diagnosis the patients were divided into five groups.Results (1)The infection rate of HPV in chronic cervicitis,chronic cervicitis with koilocystosis,low-grade squamous intraepithelial lesion(CINⅠ),high-grade squamous intraepithelial lesion(CINⅡ-Ⅲ),cervical cancer was 17.3%(13/75),70.4%(19/27),23.1%(3/13),55.6%(20/36),88.9%(8/9),respect-ively.(2)There was relationship between the genotype of HPV and the disease degree at cervix.The infection rate of single high dangerous HPV was 60.0%,in which the patients had the serious cervical disease.It is much higher than the mixed type(35.0%) and the single-low-danger type(5.0%),P<0.05.Its HPV types were 16, 18, 31, 33, 45, 56, 58 and 73.The infection rate of HPV-16 was 75.0%.Conclusion High dangerous HPV has close relationship with the disease degree at cervix.The degree of the cervical diseases was aggravated,and the rate of high dangerous HPV will increase.