中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2013年
11期
1152-1159
,共8页
郭晓姝%巩继平%杨根庆%常永丽%高莉晶%田小霞
郭曉姝%鞏繼平%楊根慶%常永麗%高莉晶%田小霞
곽효주%공계평%양근경%상영려%고리정%전소하
间充质干细胞%罗哌卡因%细胞增殖%细胞迁移
間充質榦細胞%囉哌卡因%細胞增殖%細胞遷移
간충질간세포%라고잡인%세포증식%세포천이
mesenchymal stem cell%ropivacaine%cell proliferation%cell migration
目的：探讨罗哌卡因对大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells，BMSCs)增殖能力和迁移能力的影响，为BMSCs的临床应用提供进一步的研究基础。方法：使用贴壁法分离扩增大鼠BMSCs，流式细胞仪检测细胞表面分子标志物；成脂、成骨和成肌诱导分化检测细胞的分化潜能；分别使用CCK-8和Brdu掺入方法检测不同浓度罗哌卡因处理对BMSCs增殖能力的影响；细胞划痕实验和Millicell小室迁移实验检测不同浓度罗哌卡因对大鼠BMSCs迁移能力的影响。结果：培养所得细胞具备BMSCs的表面标志物特点和多向分化潜能。50，100μmol/L罗哌卡因处理可显著降低BMSCs的增殖速度，降低BMSCs的Brdu阳性率，与对照组比较差异有统计学意义(P<0.05)。划痕实验和迁移实验表明罗哌卡因处理可引起BMSCs迁移能力的明显降低(P<0.05)。上述效应均对罗哌卡因的给药剂量呈现明显的剂量依赖关系，组间比较差异有统计学意义(P<0.05)。结论：罗哌卡因可显著降低大鼠BMSCs的增殖能力和细胞迁移能力，提示外科应用BMSCs时需注意罗哌卡因等长效局部麻醉剂对BMSCs造成的不利影响。
目的：探討囉哌卡因對大鼠骨髓間充質榦細胞(bone marrow mesenchymal stem cells，BMSCs)增殖能力和遷移能力的影響，為BMSCs的臨床應用提供進一步的研究基礎。方法：使用貼壁法分離擴增大鼠BMSCs，流式細胞儀檢測細胞錶麵分子標誌物；成脂、成骨和成肌誘導分化檢測細胞的分化潛能；分彆使用CCK-8和Brdu摻入方法檢測不同濃度囉哌卡因處理對BMSCs增殖能力的影響；細胞劃痕實驗和Millicell小室遷移實驗檢測不同濃度囉哌卡因對大鼠BMSCs遷移能力的影響。結果：培養所得細胞具備BMSCs的錶麵標誌物特點和多嚮分化潛能。50，100μmol/L囉哌卡因處理可顯著降低BMSCs的增殖速度，降低BMSCs的Brdu暘性率，與對照組比較差異有統計學意義(P<0.05)。劃痕實驗和遷移實驗錶明囉哌卡因處理可引起BMSCs遷移能力的明顯降低(P<0.05)。上述效應均對囉哌卡因的給藥劑量呈現明顯的劑量依賴關繫，組間比較差異有統計學意義(P<0.05)。結論：囉哌卡因可顯著降低大鼠BMSCs的增殖能力和細胞遷移能力，提示外科應用BMSCs時需註意囉哌卡因等長效跼部痳醉劑對BMSCs造成的不利影響。
목적：탐토라고잡인대대서골수간충질간세포(bone marrow mesenchymal stem cells，BMSCs)증식능력화천이능력적영향，위BMSCs적림상응용제공진일보적연구기출。방법：사용첩벽법분리확증대서BMSCs，류식세포의검측세포표면분자표지물；성지、성골화성기유도분화검측세포적분화잠능；분별사용CCK-8화Brdu참입방법검측불동농도라고잡인처리대BMSCs증식능력적영향；세포화흔실험화Millicell소실천이실험검측불동농도라고잡인대대서BMSCs천이능력적영향。결과：배양소득세포구비BMSCs적표면표지물특점화다향분화잠능。50，100μmol/L라고잡인처리가현저강저BMSCs적증식속도，강저BMSCs적Brdu양성솔，여대조조비교차이유통계학의의(P<0.05)。화흔실험화천이실험표명라고잡인처리가인기BMSCs천이능력적명현강저(P<0.05)。상술효응균대라고잡인적급약제량정현명현적제량의뢰관계，조간비교차이유통계학의의(P<0.05)。결론：라고잡인가현저강저대서BMSCs적증식능력화세포천이능력，제시외과응용BMSCs시수주의라고잡인등장효국부마취제대BMSCs조성적불리영향。
Objective:To observe the inlfuence of ropivacaine on the proliferation and migration of rat bone marrow mesenchymal stem cells (BMSCs) and provide basis for the clinical application of BMSCs.Methods:Rat BMSCs were isolated and cultured by adherence method. Surface markers of BMSCs were examined by lfow cytometry. Multipotent differentiation of BMSCs was detected by induced adipogenesis, osteogenesis and muscular differentiation. Proliferation of BMSCs was examined by CCK-8 and Brdu incorporation atfer ropivacaine treatment at different concentrations. Migration of BMSCs was tested by cell scratch assay and Millicell experiment.
<br> Results:Cultured cells had representative appearance and surface markers of BMSC, and they had potential multiple differentiation. Ropivacaine treatment at 50 and 100μmol/L signiifcantly reduced the proliferation rate of BMSCs and Brdu incorporation rate. There was significant difference compared with the control group (P<0.05). Cellular scratch assay and migration experiment indicated that ropivacaine significantly reduced the migration of BMSCs. There was significant difference compared with the control group (P<0.05). All these mentioned effects of ropivacaine on BMSCs were dose-dependent. There was significant difference between groups (P<0.05).
<br> Conclusion:Ropivacaine can signiifcantly reduce the proliferation and migration of rat BMSCs, suggesting that the influence of local anesthetics on BMSCs has to be taken into account when BMSCs are used in clinical practice.
