中南大学学报(医学版)
中南大學學報(醫學版)
중남대학학보(의학판)
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2013年
11期
1130-1134
,共5页
许辉%张瑾%郑理%张赛亚%成小苗
許輝%張瑾%鄭理%張賽亞%成小苗
허휘%장근%정리%장새아%성소묘
原发性高血压%肾纤维化%Notch3%锌指转录因子
原髮性高血壓%腎纖維化%Notch3%鋅指轉錄因子
원발성고혈압%신섬유화%Notch3%자지전록인자
primary hypertension%renal ifbrosis%Notch3%snail
目的:检测Notch3在原发性高血压病人和自发性高血压大鼠肾中的表达,探讨Notch3与高血压肾纤维化的关系。方法:13例原发性高血压病人为高血压病人组(HP组),15例肾肿瘤病人为对照组(CP组)。6只自发性高血压大鼠(spontaneous hypertensive rat,SHR)为高血压组(SHR组),6只同龄WKY大鼠为对照组(WKY组)。Masson三色染色法检测SHR组和WKY组肾胶原纤维的表达;免疫组织化学染色法检测Notch3在病人和大鼠肾组织中的表达;实时PCR检测锌指转录因子(snail) mRNA在SHR组和WKY组肾中的表达。结果:SHR组肾间质胶原纤维较WKY组明显增多(P<0.05);HP组Notch3的表达高于CP组(分别为6.741±0.231和0.763±0.358,P<0.001),SHR组Notch3的表达高于WKY组(分别为5.487±0.774和0.421±0.163,P<0.001);SHR组肾中snail mRNA的表达多于WKY组,差异有统计学意义(分别为0.996±0.120和0.208±0.090,P<0.01)。结论:Notch3可能与高血压肾纤维化的发生有关。
目的:檢測Notch3在原髮性高血壓病人和自髮性高血壓大鼠腎中的錶達,探討Notch3與高血壓腎纖維化的關繫。方法:13例原髮性高血壓病人為高血壓病人組(HP組),15例腎腫瘤病人為對照組(CP組)。6隻自髮性高血壓大鼠(spontaneous hypertensive rat,SHR)為高血壓組(SHR組),6隻同齡WKY大鼠為對照組(WKY組)。Masson三色染色法檢測SHR組和WKY組腎膠原纖維的錶達;免疫組織化學染色法檢測Notch3在病人和大鼠腎組織中的錶達;實時PCR檢測鋅指轉錄因子(snail) mRNA在SHR組和WKY組腎中的錶達。結果:SHR組腎間質膠原纖維較WKY組明顯增多(P<0.05);HP組Notch3的錶達高于CP組(分彆為6.741±0.231和0.763±0.358,P<0.001),SHR組Notch3的錶達高于WKY組(分彆為5.487±0.774和0.421±0.163,P<0.001);SHR組腎中snail mRNA的錶達多于WKY組,差異有統計學意義(分彆為0.996±0.120和0.208±0.090,P<0.01)。結論:Notch3可能與高血壓腎纖維化的髮生有關。
목적:검측Notch3재원발성고혈압병인화자발성고혈압대서신중적표체,탐토Notch3여고혈압신섬유화적관계。방법:13례원발성고혈압병인위고혈압병인조(HP조),15례신종류병인위대조조(CP조)。6지자발성고혈압대서(spontaneous hypertensive rat,SHR)위고혈압조(SHR조),6지동령WKY대서위대조조(WKY조)。Masson삼색염색법검측SHR조화WKY조신효원섬유적표체;면역조직화학염색법검측Notch3재병인화대서신조직중적표체;실시PCR검측자지전록인자(snail) mRNA재SHR조화WKY조신중적표체。결과:SHR조신간질효원섬유교WKY조명현증다(P<0.05);HP조Notch3적표체고우CP조(분별위6.741±0.231화0.763±0.358,P<0.001),SHR조Notch3적표체고우WKY조(분별위5.487±0.774화0.421±0.163,P<0.001);SHR조신중snail mRNA적표체다우WKY조,차이유통계학의의(분별위0.996±0.120화0.208±0.090,P<0.01)。결론:Notch3가능여고혈압신섬유화적발생유관。
Objective:To examine the expression of notch3 in the kidneys of patients with primary hypertension and rats with spontaneous hypertension, and to explore the relationship of notch3 and hypertension renal ifbrosis.Methods:hTirteen patients with primary hypertension served as a primary hypertension group (HP group), and 15 patients with kidney tumor served as a control group (CP group). The spontaneous hypertensive rats served as a primary hypertension group (SHR group, n=6), and WKY rats served as a control group (WKY group, n=6). Masson stainning was used to examine the collagen in the kidneys in the SHR group and the WKY group. Immunohistochemical staining was used to detect the levels of Notch3 in kidneys of the patients and the rats. hTe expression of snail mRNA in the kidneys in the SHR group and the WKY group was examined by real-time PCR.
<br> Results:Masson staining showed much more collagen in the SHR group than that in the WKY group (P<0.05);the expression of Notch3 in the HP group was much higher than that in the CP group ( 6.741±0.231 vs 0.763±0.358, P<0.01). The expression of Notch3 in the SHR group was much higher than that in the WKY group (5.487±0.774 vs 0.421±0.163, P<0.01), and hTe expression of snail mRNA was much higher in the SHR group than that in the WKY group (0.996±0.120 vs 0.208±0.090, P<0.01 ).
<br> Conclusion:Notch3 may be related to the occurrence of hypertension renal ifbrosis.