疑难病杂志
疑難病雜誌
의난병잡지
JOURNAL OF DIFFICULT AND COMPLICATED CASES
2014年
10期
1047-1050
,共4页
左旋多巴%结肠%酪氨酸羟化酶%ɑ-突触核蛋白%大鼠
左鏇多巴%結腸%酪氨痠羥化酶%ɑ-突觸覈蛋白%大鼠
좌선다파%결장%락안산간화매%ɑ-돌촉핵단백%대서
Levodopa%Colon%Tyrosine hydroxylase%Alpha-synuclein%Rats
目的：探讨左旋多巴（L-dopa）对帕金森病（PD）模型大鼠结肠酪氨酸羟化酶（TH）及ɑ-突触核蛋白（ɑ-Syn）表达的影响。方法健康SD大鼠72只随机分为对照组、帕金森病模型组（模型组）、左旋多巴治疗组（治疗组），各组随机再分为模型制备成功后4 d、8 d 2个亚组，各12只。采用大鼠颈背部皮下注射鱼藤酮制备帕金森病模型，免疫组化法和Westernblotting蛋白印迹法检测结肠TH和ɑ-Syn表达水平。结果1h粪便排出量及含水量：与对照组比较，模型组、治疗组大鼠显著降低（ P ＜0噜．05）；与模型组比较，治疗组大鼠增加（ P ＜0．05）；与治疗4 d亚组比较，治疗8 d亚组增加（ P ＜0．05）。免疫组化法与Western blotting检测TH、ɑ-Syn比较：（1）与对照组比较，模型组、治疗组各时间点结肠TH阳性细胞及蛋白表达均明显减少，ɑ-Syn阳性细胞及蛋白表达均明显增加（ P均＜0．05）；（2）与模型组比较，治疗组各时间点结肠TH阳性细胞及蛋白表达均增多，ɑ-Syn阳性细胞及蛋白表达均明显减少（ P均＜0．05）；（3）与治疗4 d亚组比较，治疗组治疗8 d亚组的TH阳性细胞及蛋白表达升高（ P ＜0．05），ɑ-Syn阳性细胞及蛋白降低（ P ＜0．05）。结论 PD模型大鼠结肠功能障碍的机制可能与多巴胺的减少及ɑ-Syn水平的增多有关，左旋多巴可能通过增加多巴胺和减少ɑ-Syn水平改善PD大鼠结肠功能障碍。
目的：探討左鏇多巴（L-dopa）對帕金森病（PD）模型大鼠結腸酪氨痠羥化酶（TH）及ɑ-突觸覈蛋白（ɑ-Syn）錶達的影響。方法健康SD大鼠72隻隨機分為對照組、帕金森病模型組（模型組）、左鏇多巴治療組（治療組），各組隨機再分為模型製備成功後4 d、8 d 2箇亞組，各12隻。採用大鼠頸揹部皮下註射魚籐酮製備帕金森病模型，免疫組化法和Westernblotting蛋白印跡法檢測結腸TH和ɑ-Syn錶達水平。結果1h糞便排齣量及含水量：與對照組比較，模型組、治療組大鼠顯著降低（ P ＜0嚕．05）；與模型組比較，治療組大鼠增加（ P ＜0．05）；與治療4 d亞組比較，治療8 d亞組增加（ P ＜0．05）。免疫組化法與Western blotting檢測TH、ɑ-Syn比較：（1）與對照組比較，模型組、治療組各時間點結腸TH暘性細胞及蛋白錶達均明顯減少，ɑ-Syn暘性細胞及蛋白錶達均明顯增加（ P均＜0．05）；（2）與模型組比較，治療組各時間點結腸TH暘性細胞及蛋白錶達均增多，ɑ-Syn暘性細胞及蛋白錶達均明顯減少（ P均＜0．05）；（3）與治療4 d亞組比較，治療組治療8 d亞組的TH暘性細胞及蛋白錶達升高（ P ＜0．05），ɑ-Syn暘性細胞及蛋白降低（ P ＜0．05）。結論 PD模型大鼠結腸功能障礙的機製可能與多巴胺的減少及ɑ-Syn水平的增多有關，左鏇多巴可能通過增加多巴胺和減少ɑ-Syn水平改善PD大鼠結腸功能障礙。
목적：탐토좌선다파（L-dopa）대파금삼병（PD）모형대서결장락안산간화매（TH）급ɑ-돌촉핵단백（ɑ-Syn）표체적영향。방법건강SD대서72지수궤분위대조조、파금삼병모형조（모형조）、좌선다파치료조（치료조），각조수궤재분위모형제비성공후4 d、8 d 2개아조，각12지。채용대서경배부피하주사어등동제비파금삼병모형，면역조화법화Westernblotting단백인적법검측결장TH화ɑ-Syn표체수평。결과1h분편배출량급함수량：여대조조비교，모형조、치료조대서현저강저（ P ＜0로．05）；여모형조비교，치료조대서증가（ P ＜0．05）；여치료4 d아조비교，치료8 d아조증가（ P ＜0．05）。면역조화법여Western blotting검측TH、ɑ-Syn비교：（1）여대조조비교，모형조、치료조각시간점결장TH양성세포급단백표체균명현감소，ɑ-Syn양성세포급단백표체균명현증가（ P균＜0．05）；（2）여모형조비교，치료조각시간점결장TH양성세포급단백표체균증다，ɑ-Syn양성세포급단백표체균명현감소（ P균＜0．05）；（3）여치료4 d아조비교，치료조치료8 d아조적TH양성세포급단백표체승고（ P ＜0．05），ɑ-Syn양성세포급단백강저（ P ＜0．05）。결론 PD모형대서결장공능장애적궤제가능여다파알적감소급ɑ-Syn수평적증다유관，좌선다파가능통과증가다파알화감소ɑ-Syn수평개선PD대서결장공능장애。
Objective To investigate the effect of levodopa (L-dopa) on Parkinson's disease (PD) rats colon tyrosine hydroxylase(TH)andtheexpressionofalpha-synuclein(ɑ-Syn).Methods 72healthySDratswererandomlydividedinto control group, model of PD group (model group),L-dopa treatment group (treatment group), each group was subdivided into the successful preparation of the model after 4 d, 8 d and 2 sub-groups, 12 rats in each group.Preparation of PD model rats were performed with subcutaneous injection of rotenone , immunohistochemistry and Western blotting blot were used to detect thecolonicTHandɑ-Synexpressionlevel.Results 1hfecaloutputandwatercontent:comparedwithcontrolgroup,therats in model group and the treatment group decreased significantly ( P <0.05); compared with the model group, rats in the treatment group were increased ( P <0.05); and compared with the treatment 4 d subgroups, treatment 8 d group was in-creased ( P <0.05).(1)Compared with the control group, model group and treatment group at each time point , colon TH positive cells and protein expression were significantly reduced , the expression of ɑ-Syn positive cells and protein were signifi-cantly increased ( P <0.05);(2)Compared with the model group, the treatment group at each time point, colon TH positive cells and protein expression were increased , the expression of ɑ-Syn positive cells and protein was significantly reduced ( P <0.05);(3)Compared with the 4 d treatment group, TH positive cells and protein expression was elevated in 8 d treatment subgroups ( P <0.05),ɑ-Syn positive cells and protein were decreased ( P <0.05).Conclusion The mechanism of PD model rat colonic dysfunction may related with reduced dopamine and increased ɑ-Syn level,L-dopa may by increasing dopa-mine and reduce the level of ɑ-Syn thus improve colonic dysfunction in PD rats .
