安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2013年
11期
1368-1371,1372
,共5页
王琦%于德新%徐秀民%褚晗%王大明%谢栋栋%王毅%张涛%闵捷%张志强
王琦%于德新%徐秀民%褚晗%王大明%謝棟棟%王毅%張濤%閔捷%張誌彊
왕기%우덕신%서수민%저함%왕대명%사동동%왕의%장도%민첩%장지강
精索静脉曲张%精子非整倍体%多普勒超声%荧光原位杂交%治疗
精索靜脈麯張%精子非整倍體%多普勒超聲%熒光原位雜交%治療
정색정맥곡장%정자비정배체%다보륵초성%형광원위잡교%치료
varicocele%sperm aneuploidy%CDFI%FISH%treatment
目的探讨不同程度的精索静脉曲张(VC)对精液质量和精子非整倍体率的影响及手术后的改善情况。方法34例患者经彩色多普勒超声(CDFI)诊断为VC并按不同曲张程度分为亚临床型(SVC)(n=8)、VC I级(n=13)、VCII级(n=7)和VCⅢ级(n=6)，分析精液指标，应用X、Y、18号染色体探针对其进行多色荧光原位杂交实验，检测精子染色体非整倍体数目。 VC II级和VC Ⅲ级患者行腹腔镜精索静脉高位结扎术，术后3个月复查精液指标及非整倍体数目并与术前比较。结果VC II组与VC Ⅲ组非整倍体率显著高于对照组(P<0.01)；VCII组及VCⅢ组患者行手术治疗后，精子密度、活率、活力均较术前改善(P<0.01)，非整倍体较术前显著下降(P<0.01)；Pearson相关分析显示曲张程度与非整倍体数呈正相关。结论VC导致精子染色体非整倍体增多，手术对VC II级和VC Ⅲ级患者精液质量有较明显改善作用，同时在一定程度上降低了非整倍体率。
目的探討不同程度的精索靜脈麯張(VC)對精液質量和精子非整倍體率的影響及手術後的改善情況。方法34例患者經綵色多普勒超聲(CDFI)診斷為VC併按不同麯張程度分為亞臨床型(SVC)(n=8)、VC I級(n=13)、VCII級(n=7)和VCⅢ級(n=6)，分析精液指標，應用X、Y、18號染色體探針對其進行多色熒光原位雜交實驗，檢測精子染色體非整倍體數目。 VC II級和VC Ⅲ級患者行腹腔鏡精索靜脈高位結扎術，術後3箇月複查精液指標及非整倍體數目併與術前比較。結果VC II組與VC Ⅲ組非整倍體率顯著高于對照組(P<0.01)；VCII組及VCⅢ組患者行手術治療後，精子密度、活率、活力均較術前改善(P<0.01)，非整倍體較術前顯著下降(P<0.01)；Pearson相關分析顯示麯張程度與非整倍體數呈正相關。結論VC導緻精子染色體非整倍體增多，手術對VC II級和VC Ⅲ級患者精液質量有較明顯改善作用，同時在一定程度上降低瞭非整倍體率。
목적탐토불동정도적정색정맥곡장(VC)대정액질량화정자비정배체솔적영향급수술후적개선정황。방법34례환자경채색다보륵초성(CDFI)진단위VC병안불동곡장정도분위아림상형(SVC)(n=8)、VC I급(n=13)、VCII급(n=7)화VCⅢ급(n=6)，분석정액지표，응용X、Y、18호염색체탐침대기진행다색형광원위잡교실험，검측정자염색체비정배체수목。 VC II급화VC Ⅲ급환자행복강경정색정맥고위결찰술，술후3개월복사정액지표급비정배체수목병여술전비교。결과VC II조여VC Ⅲ조비정배체솔현저고우대조조(P<0.01)；VCII조급VCⅢ조환자행수술치료후，정자밀도、활솔、활력균교술전개선(P<0.01)，비정배체교술전현저하강(P<0.01)；Pearson상관분석현시곡장정도여비정배체수정정상관。결론VC도치정자염색체비정배체증다，수술대VC II급화VC Ⅲ급환자정액질량유교명현개선작용，동시재일정정도상강저료비정배체솔。
Objective To explore the different degrees of varicocele (VC) on semen quality and sperm aneuploidy rate and improvement after surgery. Methods 34 cases of patients were diagnosed by CDFI and were divided into 4 groups according to the degree of VC:subclinical (SVC)(n=8), VC I(n=13), VC II(n=7) and VC Ⅲ(n=6). All the semen samples were routine analysis. The application of chromosomes included 18, X and Y probe in the multicolor fluorescence in situ hybridization experiment, detection of sperm aneuploidy number. VC II and VC Ⅲ level in patients with laparoscopic varicocele high ligation operation, after 3 months of semen analysis and aneuploidy number and compared with preoperative . Results There were extremely significant differences in the aneuploidy rate of VC II and VC Ⅲ ( P <0.01) compared with the control group; significant differences were found in the aneuploidy rate ( P <0.01) and the main parameters of sperm ( P <0.01) after varicocelectomy;Pearson correlation analysis showed that:varicosity degree and aneuploidy number were positively correlated. Con-clusion VC causes sperm chromosome aneuploid to increase. Surgery can make more significant improvement on semen quality in VC II and VC Ⅲ, and to some extent reduce the aneuploidy rate.
