安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2013年
11期
1355-1359
,共5页
徐秀民%于德新%张志强%谢栋栋%王毅%张涛%陈磊%闵捷%丁德茂%邹慈
徐秀民%于德新%張誌彊%謝棟棟%王毅%張濤%陳磊%閔捷%丁德茂%鄒慈
서수민%우덕신%장지강%사동동%왕의%장도%진뢰%민첩%정덕무%추자
不育症%DNA损伤%非整倍体%精子%染色体
不育癥%DNA損傷%非整倍體%精子%染色體
불육증%DNA손상%비정배체%정자%염색체
subfertile%DNA damage%aneuploidy%sperm%chromosome
目的探讨男性不育患者精子DNA完整性和染色体畸变的发生率及临床意义。方法精液标本来自正常已育男性及不育患者,以WHO标准行精液分析,根据结果及不育史分为对照组、少精症组、弱精症组、少弱精症组。精子染色质扩散实验分析精子DNA损伤率和荧光原位杂交实验分析精子染色体(18、X、Y)非整倍体率。结果少精症组、弱精症组和少弱精症组精子DNA损伤率对照组相比差异有统计学意义(P<0.01)；不育组的精子染色体(18、X、Y)非整倍体率与对照组比较差异有统计学意义(P <0.05)；精子DNA损伤率与染色体非整倍体率呈明显正相关(r=0.453, P=0.008)。结论不育患者精子DNA损伤率和染色体非整倍体率较对照组明显升高且呈显著正相关。在减数分裂过程中,精子DNA损伤可能是导致非整倍体精子形成的重要原因之一。
目的探討男性不育患者精子DNA完整性和染色體畸變的髮生率及臨床意義。方法精液標本來自正常已育男性及不育患者,以WHO標準行精液分析,根據結果及不育史分為對照組、少精癥組、弱精癥組、少弱精癥組。精子染色質擴散實驗分析精子DNA損傷率和熒光原位雜交實驗分析精子染色體(18、X、Y)非整倍體率。結果少精癥組、弱精癥組和少弱精癥組精子DNA損傷率對照組相比差異有統計學意義(P<0.01)；不育組的精子染色體(18、X、Y)非整倍體率與對照組比較差異有統計學意義(P <0.05)；精子DNA損傷率與染色體非整倍體率呈明顯正相關(r=0.453, P=0.008)。結論不育患者精子DNA損傷率和染色體非整倍體率較對照組明顯升高且呈顯著正相關。在減數分裂過程中,精子DNA損傷可能是導緻非整倍體精子形成的重要原因之一。
목적탐토남성불육환자정자DNA완정성화염색체기변적발생솔급림상의의。방법정액표본래자정상이육남성급불육환자,이WHO표준행정액분석,근거결과급불육사분위대조조、소정증조、약정증조、소약정증조。정자염색질확산실험분석정자DNA손상솔화형광원위잡교실험분석정자염색체(18、X、Y)비정배체솔。결과소정증조、약정증조화소약정증조정자DNA손상솔대조조상비차이유통계학의의(P<0.01)；불육조적정자염색체(18、X、Y)비정배체솔여대조조비교차이유통계학의의(P <0.05)；정자DNA손상솔여염색체비정배체솔정명현정상관(r=0.453, P=0.008)。결론불육환자정자DNA손상솔화염색체비정배체솔교대조조명현승고차정현저정상관。재감수분렬과정중,정자DNA손상가능시도치비정배체정자형성적중요원인지일。
Objective To investigate the changes in sperm DNA integrity and chromosomal abnormalities in some subfertile patients and its clinical significance. Methods Semen samples were obtained from healthy normal and subfertile men. All samples were classified as normal group and subfertile groups( Oligozoospermia, Asthenozo-ospermia and Oligoasthenozoospermia) according to World Health Organization guidelines. The level of DNA dam-age frequency was determined by sperm chromatin dispersion (SCD) test and the chromosomal(18,X,Y) aneu-ploidy ratio was analyzed by fluorescence in situ hybridization (FISH). Results Significant DNA damage frequen-cy differences were found among control group and subfertile groups (P<0.001);and aslo significant differences were noted in sperm chromosomal(18,X,Y) aneuploidy ratio between control group and subfertile groups (P <0.05);there were significant positive correlations between sperm DNA damage frequency and sperm chromosomal aneuploidy ratio (r=0.453,P=0.008). Conclusion In infertility patients sperm DNA damage frequency and chromosome aneuploidy ratio are obviously higher than normal control group and has significant positive correlation. In the process of meiosis, DNA damage may be the risk factor causing sperm chromosomal aneuploidy.
