CAJ | 학술논문

目的探讨在不同处理条件下生成的钛酸钙(CaT-iO3)涂覆的纯钛片,对成骨细胞生长的影响。方法利用不同条件下水热合成法制备CaTiO3涂覆的纯钛片,通过扫描电镜(SEM)、X射线衍射(XRD)对涂覆前后的钛片进行分析；将成骨细胞与材料共培养,利用显微镜观察法分别观察成骨细胞在不同处理材料表面的铺展状态,采用考马斯亮蓝法测定相对蛋白质含量、碱性磷酸酶(ALP)活性,并进行统计学分析。结果CaTiO3涂覆的纯钛片的相对蛋白质含量和ALP活性,与对照组(无CaTiO3涂覆的纯钛片)比较,差异有统计学意义(P<0.05,P<0.01)。但CaTiO3涂覆的纯钛片在120、150℃和不同时间处理后对成骨细胞ALP活性及相对蛋白质含量未见明显改变。结论成骨细胞离体条件下,在120、150℃和不同时间处理后CaTiO3涂覆的纯钛片对成骨细胞的增殖和分化有促进作用,对ALP活性和相对蛋白质含量具有增高的影响,CaTiO3涂覆的纯钛片表面更有利于成骨细胞的黏附、增殖且对成骨细胞无毒性作用。
목적탐토재불동처리조건하생성적태산개(CaT-iO3)도복적순태편,대성골세포생장적영향。방법이용불동조건하수열합성법제비CaTiO3도복적순태편,통과소묘전경(SEM)、X사선연사(XRD)대도복전후적태편진행분석；장성골세포여재료공배양,이용현미경관찰법분별관찰성골세포재불동처리재료표면적포전상태,채용고마사량람법측정상대단백질함량、감성린산매(ALP)활성,병진행통계학분석。결과CaTiO3도복적순태편적상대단백질함량화ALP활성,여대조조(무CaTiO3도복적순태편)비교,차이유통계학의의(P<0.05,P<0.01)。단CaTiO3도복적순태편재120、150℃화불동시간처리후대성골세포ALP활성급상대단백질함량미견명현개변。결론성골세포리체조건하,재120、150℃화불동시간처리후CaTiO3도복적순태편대성골세포적증식화분화유촉진작용,대ALP활성화상대단백질함량구유증고적영향,CaTiO3도복적순태편표면경유리우성골세포적점부、증식차대성골세포무독성작용。
Objective To evaluate the impact of Calcium Titanate ( CaTiO3 ) coated pure titanium on the growth of osteoblasts. Methods The uniform CaTiO3 coatings on Ti substrates, prepared by a simple hydrothermal synthesis method, which was characterized by means of SEM and XRD before and after the modification. Spreadability status of osteoblasts on the surface of the different treatment materials was observed with microscope. The determination of relative protein content (Coomassie blue), alkaline phosphatase (ALP) activity of osteoblasts was measured by co-culture with materials and analysized statistically. Results The protein content and alkaline phosphatase activity of CaTiO3 coated titanium piece at different temperature and time, as compared with the control group (no CaTiO3 coating of titanium), the difference had statistical significance ( P <0.05,P <0.01). CaTiO3 coating titanium sheet processed at 120, 150 ℃ temperature and different time had no significant changes on the ALP activity and relative protein content. Conclusion The CaTiO3 coated titanium piece, after processed at 120, 150 ℃ for differ-ent time, has a promotion effect on the proliferation and differentiation of osteoblasts under isolated conditions and improved ALP activity and relative protein content. Calcium Titanate coated titanium sheet surface is more condu-cive to osteoblasts adhesion, proliferation without toxic effects on osteoblasts.