CAJ | 학술논문

目的观察不同浓度维生素C(Vit C)对膀胱癌T-24细胞增殖和凋亡的影响,并了解其可能的机制。方法使用不同浓度Vit C干预体外培养的膀胱癌T-24细胞,然后通过缩胆囊素八肽(CCK-8)检测T-24细胞增殖情况,用流式细胞术检测细胞周期的变化及其凋亡率结果,RT-PCR法检测Survivin mRNA、Caspase-3 mRNA的表达。结果400μg/ml Vit C可将T-24细胞阻滞于S期,诱导其凋亡并且下调Sur-vivin mRNA 的表达、上调 Caspase-3 mRNA 的表达。4、40μg/ml的Vit C对人膀胱癌T-24细胞无明显抑制增殖作用,而400μg/ml Vit C对人膀胱癌T-24细胞增殖有显著抑制作用。结论400μg/ml Vit C可抑制T-24细胞增殖、诱导其凋亡,其机制可能与下调Survivin mRNA表达、上调Caspase-3 mRNA表达有关。
목적관찰불동농도유생소C(Vit C)대방광암T-24세포증식화조망적영향,병료해기가능적궤제。방법사용불동농도Vit C간예체외배양적방광암T-24세포,연후통과축담낭소팔태(CCK-8)검측T-24세포증식정황,용류식세포술검측세포주기적변화급기조망솔결과,RT-PCR법검측Survivin mRNA、Caspase-3 mRNA적표체。결과400μg/ml Vit C가장T-24세포조체우S기,유도기조망병차하조Sur-vivin mRNA 적표체、상조 Caspase-3 mRNA 적표체。4、40μg/ml적Vit C대인방광암T-24세포무명현억제증식작용,이400μg/ml Vit C대인방광암T-24세포증식유현저억제작용。결론400μg/ml Vit C가억제T-24세포증식、유도기조망,기궤제가능여하조Survivin mRNA표체、상조Caspase-3 mRNA표체유관。
Objective To observe the effects of different concentrations of vitamin C (Vit C) on the proliferation and apoptosis in bladder cancer cell T-24 cells and explore its possible mechanism. Methods Different concentra-tions of Vit C were used to intervene in vitro T-24 cells. Cholecystokinin Octapeptolecyst (CCK-8) was detected to show the proliferous situation of T-24 cells. Flow cytometry was used to detect cell cycle changes and apoptosis rate and RT-PCR was used to detect the expressions of Survivin mRNA and Caspase-3 mRNA. Results 400μg/ml Vit C blocked T-24 cells in S phase, induced the apoptosis, reduced the Survivin mRNA expression and raised the Caspase-3 mRNA expression. 4 μg/ml and 40 μg/ml concentrations of Vit C did not play a significant inhibitory role in the proliferation in T-24 cells but 400 μg/ml of Vit C had significant inhibitory effects on the proliferation of T-24 cells. Conclusion 400 μg/ml Vit C can inhibit the proliferation of T-24 cells and induce the apoptosis. The possible mechanism is that Vit C reduces the expressions of Survivin mRNA, and promotes the expression of Caspase-3 mRNA.