浙江临床医学
浙江臨床醫學
절강림상의학
ZHEJIANG CLINICAL MEDICAL JOURNAL
2013年
12期
1784-1786,1787
,共4页
郑如莲%杨鹏麟%陈如杰%李继武
鄭如蓮%楊鵬麟%陳如傑%李繼武
정여련%양붕린%진여걸%리계무
肾上腺髓质素2%小分子多肽%CRLR/RAMPS受体系统%肾性高血压
腎上腺髓質素2%小分子多肽%CRLR/RAMPS受體繫統%腎性高血壓
신상선수질소2%소분자다태%CRLR/RAMPS수체계통%신성고혈압
Intermedin%micromolecular%CRLR/RAMPS receptor system%Renal hypertension
目的：通过观察小分子生物活性肽肾上腺髓质素2在大鼠两肾一夹术后肾性高血压发生、发展过程中的变化，探讨其在病理、生理过程中的作用。方法清洁级雄性SD大鼠18只，体重180~220g，随机分为模型组和对照组。模型组行两肾一夹手术，对照组仅分离肾动脉，8周后作左心导管插管法测定血流动力学指标，并计算左室重量与体重比值；采用逆转录-聚合酶链反应方法测定心肌肾上腺髓质素2的mRNA含量变化；采用免疫组织化学方法测定左心室心肌组织的肾上腺髓质素2表达，并对表达强度进行半定量分析。结果模型组大鼠颈动脉收缩压、平均颈动脉压、LV±dp/dtmax、LVEDP较对照组明显升高（P均<0.01），左室重量与体重也升高（P<0.05）；模型组大鼠左室心肌肾上腺髓质素2的mRNA表达较对照组高（P<0.01），免疫组化的累积光密度较对照组升高95%（P<0.01），且主要发布在心肌细胞的细胞核。结论肾上腺髓质素2在肾性高血压大鼠的心肌内基因表达上调及蛋白合成增加，提示其在肾性高血压致心肌肥厚的过程中起重要作用。
目的：通過觀察小分子生物活性肽腎上腺髓質素2在大鼠兩腎一夾術後腎性高血壓髮生、髮展過程中的變化，探討其在病理、生理過程中的作用。方法清潔級雄性SD大鼠18隻，體重180~220g，隨機分為模型組和對照組。模型組行兩腎一夾手術，對照組僅分離腎動脈，8週後作左心導管插管法測定血流動力學指標，併計算左室重量與體重比值；採用逆轉錄-聚閤酶鏈反應方法測定心肌腎上腺髓質素2的mRNA含量變化；採用免疫組織化學方法測定左心室心肌組織的腎上腺髓質素2錶達，併對錶達彊度進行半定量分析。結果模型組大鼠頸動脈收縮壓、平均頸動脈壓、LV±dp/dtmax、LVEDP較對照組明顯升高（P均<0.01），左室重量與體重也升高（P<0.05）；模型組大鼠左室心肌腎上腺髓質素2的mRNA錶達較對照組高（P<0.01），免疫組化的纍積光密度較對照組升高95%（P<0.01），且主要髮佈在心肌細胞的細胞覈。結論腎上腺髓質素2在腎性高血壓大鼠的心肌內基因錶達上調及蛋白閤成增加，提示其在腎性高血壓緻心肌肥厚的過程中起重要作用。
목적：통과관찰소분자생물활성태신상선수질소2재대서량신일협술후신성고혈압발생、발전과정중적변화，탐토기재병리、생리과정중적작용。방법청길급웅성SD대서18지，체중180~220g，수궤분위모형조화대조조。모형조행량신일협수술，대조조부분리신동맥，8주후작좌심도관삽관법측정혈류동역학지표，병계산좌실중량여체중비치；채용역전록-취합매련반응방법측정심기신상선수질소2적mRNA함량변화；채용면역조직화학방법측정좌심실심기조직적신상선수질소2표체，병대표체강도진행반정량분석。결과모형조대서경동맥수축압、평균경동맥압、LV±dp/dtmax、LVEDP교대조조명현승고（P균<0.01），좌실중량여체중야승고（P<0.05）；모형조대서좌실심기신상선수질소2적mRNA표체교대조조고（P<0.01），면역조화적루적광밀도교대조조승고95%（P<0.01），차주요발포재심기세포적세포핵。결론신상선수질소2재신성고혈압대서적심기내기인표체상조급단백합성증가，제시기재신성고혈압치심기비후적과정중기중요작용。
Aim To study the changes and probable role of intermedin/adrenomedullin 2(IMD/ADM2),a novel micromolecular bioactive peptide,in rats with Two-kidney,One-clip renal hypertension. Methods Eighteen healthy male Sprague-Dawley(SD)rats(180~220g)were randomly divided into normal control group and model group. The rats of model group were cliped with one renal artery,and the contral group only separation one renal artery. After 8 weeks,the left carotid artery was cannulated with a catheter and connected to apressure transducer to monitor the SBP,DBP,mCAP,LVEDP and LV±dp/dtmax. Meanwhile,the LVM/BW was also measured. IMD/ADM2 mRNA expressed in the left ventricular heart muscles were studied with reverse transcription-polymerase chain reaction(RT-PCR). The location and distribution were detected in the left ventricular heart muscles by using immunohistochemical(IHC),and semiquantitative analysis was performed in positive regions by using special software-Image Pro Plus(IPP). Results The systolic blood pressure,mean carotid artery pressure,maximum left ventricular developed pressure increase or decreased rate(±dp/dtmax)and LVEDP of 2K1C group were higher than those of normal control group significantly(all P<0.01);The LVM/BW of 2K1C group were higher than those of normal control group(P<0.05);The expression of IMD/ADM2 mRNA of the model group were increased(P<0.01)comparing to control group in the left ventricular organization respectively. The cumulative light density of IMD/ADM2 in the left ventricular organization showed that the model group was higher(P<0.01)than control group,and the main expression of IMD/ADM2 in myocardial issues were cell nuclei,inflammatory cells. Conclusion These results confirm IMD/ADM2 played an important role in the process of renal hypertension by the mRNA expression and the cumulative light density of IMD/ADM2 are increased In the left ventricular heart muscles of the renal Hypertension rats.
