国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2013年
12期
2414-2417
,共4页
单纯疱疹病毒性角膜炎%诊断%实验室检测技术
單純皰疹病毒性角膜炎%診斷%實驗室檢測技術
단순포진병독성각막염%진단%실험실검측기술
herpes simplexkeratitis%diagnosis%laboratory techniques
单纯疱疹病毒性角膜( herpes simplex keratitis , HSK )是一种严重的感染性致盲性眼病。过去HSK的诊断主要依据临床特征,然而这并不可靠。随着分子生物学的发展,目前对于单纯疱疹病毒性角膜炎的诊断有了突飞猛进的发展。共焦显微技术、环媒恒温扩增技术、实时荧光探针定量聚合酶链式反应( polymerase chain reaction , PCR )、巢式PCR(nested PCR)及多重聚合酶链式反应(multiplex PCR)等被认为是较敏感且快速的诊断方法。
單純皰疹病毒性角膜( herpes simplex keratitis , HSK )是一種嚴重的感染性緻盲性眼病。過去HSK的診斷主要依據臨床特徵,然而這併不可靠。隨著分子生物學的髮展,目前對于單純皰疹病毒性角膜炎的診斷有瞭突飛猛進的髮展。共焦顯微技術、環媒恆溫擴增技術、實時熒光探針定量聚閤酶鏈式反應( polymerase chain reaction , PCR )、巢式PCR(nested PCR)及多重聚閤酶鏈式反應(multiplex PCR)等被認為是較敏感且快速的診斷方法。
단순포진병독성각막( herpes simplex keratitis , HSK )시일충엄중적감염성치맹성안병。과거HSK적진단주요의거림상특정,연이저병불가고。수착분자생물학적발전,목전대우단순포진병독성각막염적진단유료돌비맹진적발전。공초현미기술、배매항온확증기술、실시형광탐침정량취합매련식반응( polymerase chain reaction , PCR )、소식PCR(nested PCR)급다중취합매련식반응(multiplex PCR)등피인위시교민감차쾌속적진단방법。
Herpes simplex keratitis ( HSK) is the leading cause of infectious blindness. In general, diagnosis of HSK is based on clinical findings, which often are not confirmatory. With the development of molecular biology, a variety of laboratory methods that helped in achieving a diagnosis including in vivo confocal microscopy ( IVCM ) , loop - mediated isothermal amplification ( LAMP ) , real -time polymerase chain reaction (rt-PCR), nested PCR and multiplex PCR.They are considered as accurate and fast diagnostic techniques, thus characteristics of these are reviewed in this paper.