国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2013年
12期
2385-2389
,共5页
倍频532 nm激光%视网膜激光损伤%组织病理学
倍頻532 nm激光%視網膜激光損傷%組織病理學
배빈532 nm격광%시망막격광손상%조직병이학
frequency-doubled 532nm laser%laser-induced retinal damage%histopathology
目的：通过倍频532 nm激光照射青紫蓝兔眼底，观察不同时期视网膜光斑区域组织形态学改变。<br> 方法：健康青紫蓝兔20只随机分为A组16只（激光损伤组）和B组4只（正常对照组），使用倍频532 nm激光照射A组兔视网膜（光斑大小200μm，脉宽0．05s，光斑TsoⅢ），B组未行光凝。按激光光凝术后不同时相点：1d；1，2 wk；1 mo观察眼底照片的改变、光镜下组织形态学改变、电镜下超微结构的改变及光斑直径在不同时相点的变化。<br> 结果：（1）眼底照相：随时间推移，光斑由术后1d时的浓白色水肿外观变为术后1mo时的黑色素附着区域；（2）光镜观察：光凝后光斑区域主要损伤集中在视网膜外层，光感受器细胞坏死、凋亡，随时间推移，胶质细胞、色素细胞充填损伤区域，形成纤维增殖；（3）电镜观察：光凝后出现明显的视细胞外节膜盘结构模糊，排列紊乱，线粒体嵴模糊、断裂甚至消失，明显的空泡样变，术后1 wk开始即出现细胞染色质集边等凋亡改变，后期胶原纤维增殖；（4）光斑大小：光斑大小从术后1d时的最大值随时间推移，逐渐变小，术后1 wk时光斑大小缩小15％，术后2 wk时光斑大小缩小20％，术后1 mo时光斑大小缩小21％。结论：倍频532 nm激光照射青紫蓝兔黄斑区，光斑反应术后1 d时最为明显，至术后2 wk左右基本稳定。
目的：通過倍頻532 nm激光照射青紫藍兔眼底，觀察不同時期視網膜光斑區域組織形態學改變。<br> 方法：健康青紫藍兔20隻隨機分為A組16隻（激光損傷組）和B組4隻（正常對照組），使用倍頻532 nm激光照射A組兔視網膜（光斑大小200μm，脈寬0．05s，光斑TsoⅢ），B組未行光凝。按激光光凝術後不同時相點：1d；1，2 wk；1 mo觀察眼底照片的改變、光鏡下組織形態學改變、電鏡下超微結構的改變及光斑直徑在不同時相點的變化。<br> 結果：（1）眼底照相：隨時間推移，光斑由術後1d時的濃白色水腫外觀變為術後1mo時的黑色素附著區域；（2）光鏡觀察：光凝後光斑區域主要損傷集中在視網膜外層，光感受器細胞壞死、凋亡，隨時間推移，膠質細胞、色素細胞充填損傷區域，形成纖維增殖；（3）電鏡觀察：光凝後齣現明顯的視細胞外節膜盤結構模糊，排列紊亂，線粒體嵴模糊、斷裂甚至消失，明顯的空泡樣變，術後1 wk開始即齣現細胞染色質集邊等凋亡改變，後期膠原纖維增殖；（4）光斑大小：光斑大小從術後1d時的最大值隨時間推移，逐漸變小，術後1 wk時光斑大小縮小15％，術後2 wk時光斑大小縮小20％，術後1 mo時光斑大小縮小21％。結論：倍頻532 nm激光照射青紫藍兔黃斑區，光斑反應術後1 d時最為明顯，至術後2 wk左右基本穩定。
목적：통과배빈532 nm격광조사청자람토안저，관찰불동시기시망막광반구역조직형태학개변。<br> 방법：건강청자람토20지수궤분위A조16지（격광손상조）화B조4지（정상대조조），사용배빈532 nm격광조사A조토시망막（광반대소200μm，맥관0．05s，광반TsoⅢ），B조미행광응。안격광광응술후불동시상점：1d；1，2 wk；1 mo관찰안저조편적개변、광경하조직형태학개변、전경하초미결구적개변급광반직경재불동시상점적변화。<br> 결과：（1）안저조상：수시간추이，광반유술후1d시적농백색수종외관변위술후1mo시적흑색소부착구역；（2）광경관찰：광응후광반구역주요손상집중재시망막외층，광감수기세포배사、조망，수시간추이，효질세포、색소세포충전손상구역，형성섬유증식；（3）전경관찰：광응후출현명현적시세포외절막반결구모호，배렬문란，선립체척모호、단렬심지소실，명현적공포양변，술후1 wk개시즉출현세포염색질집변등조망개변，후기효원섬유증식；（4）광반대소：광반대소종술후1d시적최대치수시간추이，축점변소，술후1 wk시광반대소축소15％，술후2 wk시광반대소축소20％，술후1 mo시광반대소축소21％。결론：배빈532 nm격광조사청자람토황반구，광반반응술후1 d시최위명현，지술후2 wk좌우기본은정。
AIM: To observe histopathology changes of spot regional in chinchilla rabbit's retina at different periods after frequency-doubled 532nm laser irradiation. <br> METHODS: Twenty healthy rabbits were randomly divided into two groups: 16 in group A ( laser group ) and 4 in group B (normal control group).According to different time after laser photocoagulation rabbits were divided into four subgroups: 1d, 1 week, 2 weeks, 1month, group A with 4 for each subgroup;group B with 1 for each subgroup. To observe the histopathologic changes we used fundus photograph, light microscope, electron microscope and spot diameter calculating in different time points. <br> RESULTS: ( 1 ) Fundus photography: the spot region changed from thick white edema appearance on 1d after the surgery to melanoma attachment by 1 month; ( 2 ) Light microscopy: the main damage spot region was concentrated in the outer retina after photocoagulation. Photoreceptor cell appeared necrosis and apoptosis. With the passage of time, glial cells, pigment cells filled injury regional, and fibrous proliferation was formed;( 3 ) Electron microscope: after photocoagulation, structure of membranous disc blurred and arranged in disorder; mitochondrial cristae became vague, fracture or even disappear; obvious vacuolization appeared.It began to appear apoptosis after 1 week, and gradually the fibroblast proliferation appeared; ( 4 ) Spot size: the spot size was the maximum at 1d, then gradually became smaller, spot size down to 85% at one-week time, 80%at 2-week time, and 79%at one-month time. CONCLUSION: Frequency -doubled 532nm laser irradiation of pigmented macular, spot reaction is most obvious at 1d and stabilized at about 2 weeks.
