CAJ | 학술논문

目的：通过分析儿童眼肌型重症肌无力患儿外周血中免疫球蛋白、补体及T细胞亚群的含量，初步探讨体液免疫和细胞免疫在儿童眼肌型重症肌无力发病中的作用。 　　方法：采用免疫比浊法检测37例儿童眼肌型重症肌无力患儿及健康对照儿童外周血中IgG，IgA，IgM，补体C3及C4的含量，流式细胞术检测12例儿童眼肌型重症肌无力患儿及健康对照儿童外周血中CD3＋T淋巴细胞、CD4＋T淋巴细胞及CD8＋T淋巴细胞的含量，独立样本t检验进行数据统计分析。 　　结果：儿童眼肌型重症肌无力患儿外周血 IgA， IgM 及CD3＋T淋巴细胞、CD8＋T淋巴细胞值与健康儿童比较，均无显著性差异（P＞0．05）， IgG，补体C3及C4的含量低于健康儿童（ P＜0．05）。 CD4＋T淋巴细胞值高于对照儿童（P＜0．05）。 　　结论：补体C3， C4及CD4＋T淋巴细胞在儿童眼肌型重症肌无力的发病机制中起着重要作用。
목적：통과분석인동안기형중증기무력환인외주혈중면역구단백、보체급T세포아군적함량，초보탐토체액면역화세포면역재인동안기형중증기무력발병중적작용。 　　방법：채용면역비탁법검측37례인동안기형중증기무력환인급건강대조인동외주혈중IgG，IgA，IgM，보체C3급C4적함량，류식세포술검측12례인동안기형중증기무력환인급건강대조인동외주혈중CD3＋T림파세포、CD4＋T림파세포급CD8＋T림파세포적함량，독립양본t검험진행수거통계분석。 　　결과：인동안기형중증기무력환인외주혈 IgA， IgM 급CD3＋T림파세포、CD8＋T림파세포치여건강인동비교，균무현저성차이（P＞0．05）， IgG，보체C3급C4적함량저우건강인동（ P＜0．05）。 CD4＋T림파세포치고우대조인동（P＜0．05）。 　　결론：보체C3， C4급CD4＋T림파세포재인동안기형중증기무력적발병궤제중기착중요작용。
AIM: To discuss the role of humoral immunity and cellular immunity in the development of ocular myasthenia gravis in children by analyzing the clinical value of the indices including immunoglobulin, complement and T cell subgroup in peripheral blood. METHODS: The concentrations of serum IgG, IgA, IgM, C3 and C4 in the myasthenia gravis group and the control group were detected by immune compare turbid. The contents of CD3+T cell, CD4+T cell and CD8+T cell were detected by flow cytometry.Data was analyzed by Independent-Sample Test. RESULTS: There were no significant differences in contents of IgA, IgM, CD3+T cell, CD8+T cell between ocular myasthenia gravis group in children and the control group ( P>0.05).The concentrations of serum IgG, C3 and C4 for myasthenia gravis group were lower than those of the control group (P<0.05).The content of CD4+T cell were higher than those of the control group (P<0.05). CONCLUSION: Complement C3, C4 and CD4+T cell played an important role in immunology pathogenesis mechanism for ocular myasthenia gravis in children.