CAJ | 학술논문

目的探讨低分子量酪氨酸磷酸酶(LMW-PTP)在子宫颈鳞癌中的表达及其临床意义。方法采用RT-PCR及免疫组化法分析HaCaT、C33A、SiHa和CaSki细胞系中LMW-PTP的表达情况。免疫组织化学法检测正常宫颈患者19例，宫颈上皮内瘤变(CIN)I期患者18例、CIN II~Ⅲ患者46例和宫颈鳞癌患者76例的组织切片中 LMW-PTP的表达。结果LMW-PTP mRNA及蛋白在 C33A、SiHa及 CaSki宫颈癌细胞系中表达明显高于人永生化正常表皮细胞系HaCaT；临床组织样本中，正常宫颈上皮LMW-PTP几乎不表达，在正常宫颈上皮到CIN继而发展成宫颈癌的进展过程中LMW-PTP的表达逐渐升高，差异有统计学意义(P<0.05)；低分化宫颈癌组织中LMW-PTP表达高于中分化组织(P=0.025)，中分化宫颈癌组织中LMW-PTP的表达高于高分化组织(P=0.045)；宫颈癌组织中LMW-PTP蛋白的表达与组织学分级程度和是否有淋巴结转移密切相关(P<0.05)。结论LMW-PTP在宫颈癌的进展过程中起重要作用，且与组织分化程度和是否有淋巴转移密切相关，提示LMW-PTP的表达有助于判断宫颈癌患者的预后。
목적탐토저분자량락안산린산매(LMW-PTP)재자궁경린암중적표체급기림상의의。방법채용RT-PCR급면역조화법분석HaCaT、C33A、SiHa화CaSki세포계중LMW-PTP적표체정황。면역조직화학법검측정상궁경환자19례，궁경상피내류변(CIN)I기환자18례、CIN II~Ⅲ환자46례화궁경린암환자76례적조직절편중 LMW-PTP적표체。결과LMW-PTP mRNA급단백재 C33A、SiHa급 CaSki궁경암세포계중표체명현고우인영생화정상표피세포계HaCaT；림상조직양본중，정상궁경상피LMW-PTP궤호불표체，재정상궁경상피도CIN계이발전성궁경암적진전과정중LMW-PTP적표체축점승고，차이유통계학의의(P<0.05)；저분화궁경암조직중LMW-PTP표체고우중분화조직(P=0.025)，중분화궁경암조직중LMW-PTP적표체고우고분화조직(P=0.045)；궁경암조직중LMW-PTP단백적표체여조직학분급정도화시부유림파결전이밀절상관(P<0.05)。결론LMW-PTP재궁경암적진전과정중기중요작용，차여조직분화정도화시부유림파전이밀절상관，제시LMW-PTP적표체유조우판단궁경암환자적예후。
Objective To detect the expression of low molecular weight protein tyrosine phosphatase (LMW-PTP) proteins and its clinical significance in cervical cancer. Methods The expressions of LMW-PTP were determined by RT-PCR and immunohistochemical assay in HaCaT, C33A, SiHa and CaSki. A series of samples, including 19 cases of normal cervical tissues, 18 cases of cervical intraepithelial neoplasia I phase, 46 cases of cervical intraepi-thelial neoplasia II or Ⅲ phase, 76 cases of squamous cell carcinoma (SCC), were used to examine LMW-PTP immunohistochemical staining and statistical analysis. Results RT-PCR and Western blot results showed that ex-pression of LMW-PTP in C33A, SiHa and CaSki were higher than expression of LMW-PTP in HaCaT. The results showed that LMW-PTP was barely observed in the normal cervical epithelial cells. With the progression of normal cervical epithelial cells to CIN and cervical cancer, the expression levels of LMW-PTP were increased. There was significantly difference for LMW-PTP expression among normal cervical tissues, CIN, cervical cancer (P<0.05). 20 The expression of LMW-PTP in poorly differentiated cervical cancer was higher than expression of LMW-PTP in moderately differentiated cervical cancer which was higher expression of LMW-PTP than high differentiation of cer-vical cancer (P=0.025, P=0.045, respectively). Overexpression of LMW-PTP were related to lymph node me-tastasis and histological grade of SCC(P<0.05). Conclusion The results suggest that LMW-PTP are involved with the progression of cervical carcinogenesis and development of cervical cancer. In addition, the association LMW-PTP with lymph node metastasis and histological grade of SCC suggests that LMW-PTP can be a potential therapeutic target of SCC patients.