CAJ | 학술논문

目的检测类风湿关节炎(RA)患者血清中白介素(IL)-32及趋化因子CXCL13的水平,以探讨其在RA中的意义。方法采用酶联免疫吸附试验(ELISA)法测定102例RA患者和50例正常对照者血清中 IL-32及趋化因子CXCL13水平。同时测定RA患者X线片分期、骨质破坏程度、关节压痛数、关节肿胀数、疾病活动度评分(DAS28)、红细胞沉降率( ESR)、C 反应蛋白( CRP)、抗环瓜氨酸肽(CCP)抗体、类风湿因子(RF)、肿瘤坏死因子(TNF)-α、IL-6等各种临床及炎症指标,进行相关性分析。结果RA患者血清中IL-32及趋化因子CXCL13水平明显高于正常对照者(P<0.05),且严重骨质破坏组 IL-32及趋化因子 CXCL13水平高于轻度或无骨质破坏组(P<0.05)；IL-32及趋化因子CXCL13水平均与RA患者X线片分期、骨质破坏程度、DAS28、ESR、CRP、CCP、TNF-α、IL-6呈正相关,且RA患者血清中 IL-32及趋化因子 CXCL13水平之间呈正相关( r =0.509,P=0.000),而与RA患者关节肿胀数、关节压痛数和血清中RF无相关性。结论RA患者血清中IL-32及趋化因子CXCL13与疾病活动度及相关炎症指标明显相关,提示IL-32及趋化因子CXCL13与RA存在密切联系,可作为RA判断病情及疾病活动的新指标。
목적검측류풍습관절염(RA)환자혈청중백개소(IL)-32급추화인자CXCL13적수평,이탐토기재RA중적의의。방법채용매련면역흡부시험(ELISA)법측정102례RA환자화50례정상대조자혈청중 IL-32급추화인자CXCL13수평。동시측정RA환자X선편분기、골질파배정도、관절압통수、관절종창수、질병활동도평분(DAS28)、홍세포침강솔( ESR)、C 반응단백( CRP)、항배과안산태(CCP)항체、류풍습인자(RF)、종류배사인자(TNF)-α、IL-6등각충림상급염증지표,진행상관성분석。결과RA환자혈청중IL-32급추화인자CXCL13수평명현고우정상대조자(P<0.05),차엄중골질파배조 IL-32급추화인자 CXCL13수평고우경도혹무골질파배조(P<0.05)；IL-32급추화인자CXCL13수평균여RA환자X선편분기、골질파배정도、DAS28、ESR、CRP、CCP、TNF-α、IL-6정정상관,차RA환자혈청중 IL-32급추화인자 CXCL13수평지간정정상관( r =0.509,P=0.000),이여RA환자관절종창수、관절압통수화혈청중RF무상관성。결론RA환자혈청중IL-32급추화인자CXCL13여질병활동도급상관염증지표명현상관,제시IL-32급추화인자CXCL13여RA존재밀절련계,가작위RA판단병정급질병활동적신지표。
Objective To measure the serum levels of IL-32 and chemokine CXCL13 in patients with rheumatoid arthritis and explore their clinical value. Methods The serum levels of IL-32 and chemokine CXCL13 were meas-ured by ELISA methods in 102 patients with RA and 50 healthy controls;analyzed the correlation among the IL-32, chemokine CXCL13 and the clinical data, inflammation maker of RA, such as X-ray stage, the bone destruction, the number of tender joints, the number of swollen joints, DAS28,ESR,CRP,CCP,RF,TNF-α,IL-6, etc. Results The serum levels of IL-32 and chemokine CXCL13 were significantly higher in RA patients than that in healthy controls(P<0.05). Moreover,the serum levels of IL-32 and chemokine CXCL13 those patients who had bone de-struction were higher than those with no or mild bone destructions(P<0.05);there was a positive correlation be-tween IL-32, chemokine CXCL13 and X-ray stage, the bone destruction, DAS28, ESR,CRP,CCP,TNF-α,IL-6. Especially, there was a positive correlation between IL-32 and chemokine CXCL13 (r=0.509,P=0.000). How-ever, there was no relationship with the number of swollen joints, tender joints, and RF. Conclusion The serum levels of IL-32 and chemokine CXCL13 were increased significantly,which was closly related with the disease activ-ity and inflammation maker. The results indicate that the abnormality of IL-32 and chemokine CXCL13 may be use-ful for the evaluation of disease activity and monitoring the progress of the disease.