安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2013年
12期
1484-1487
,共4页
卞秀梅%吴正祥%陶科明%李为慧
卞秀梅%吳正祥%陶科明%李為慧
변수매%오정상%도과명%리위혜
溃疡性结肠炎%吲哚胺2,3-双加氧酶%犬尿氨酸%ROC曲线
潰瘍性結腸炎%吲哚胺2,3-雙加氧酶%犬尿氨痠%ROC麯線
궤양성결장염%신타알2,3-쌍가양매%견뇨안산%ROC곡선
ulcerative colitis%indoleamine 2,3-dioxygenase%kynurenine%ROC curve
目的检测吲哚胺2,3-双加氧酶(IDO)和犬尿氨酸(KYN)在溃疡性结肠炎(UC)患者中的表达水平,利用受试者工作特征(ROC)曲线分析血清IDO、KYN诊断活动期UC的价值。方法60例UC组、18例疾病对照组和20例健康对照组。采用免疫组化法检测UC组结肠黏膜中IDO的表达,ELISA法检测3组血清中 IDO、KYN 和 C 反应蛋白(CRP)水平,利用ROC曲线进行分析。结果① UC组血清中IDO和KYN水平与结肠黏膜IDO表达量呈正相关(P<0.05)；② UC组患者血清IDO及KYN水平高②疾病对照组和健康对照组(P<0.05)；③ UC组中,活动期患者血清IDO、KYN水平高②缓解期(P<0.01),并随炎症严重程度升高而升高；④活动期UC组IDO、KYN水平与疾病活动度、CRP水平呈正相关；⑤ IDO、KYN、CRP的ROC曲线下面积分别为0.976、0.856和0.864；最佳截断值分别为53.66 U/L、2.34 nmol/L 和1.75 mg/ml；灵敏度分别为90.5%、78.65%和100.0%；特异度分别为94.4%、83.3%和61.1%；Youden指数分别为0.8492、0.6190和0.6111。结论UC患者血清IDO和KYN水平可以作为判断UC患者炎症程度的重要指标,血清IDO、KYN和CRP水平对活动期UC有重要的诊断价值,其中 IDO 诊断价值高② KYN 和CRP。
目的檢測吲哚胺2,3-雙加氧酶(IDO)和犬尿氨痠(KYN)在潰瘍性結腸炎(UC)患者中的錶達水平,利用受試者工作特徵(ROC)麯線分析血清IDO、KYN診斷活動期UC的價值。方法60例UC組、18例疾病對照組和20例健康對照組。採用免疫組化法檢測UC組結腸黏膜中IDO的錶達,ELISA法檢測3組血清中 IDO、KYN 和 C 反應蛋白(CRP)水平,利用ROC麯線進行分析。結果① UC組血清中IDO和KYN水平與結腸黏膜IDO錶達量呈正相關(P<0.05)；② UC組患者血清IDO及KYN水平高②疾病對照組和健康對照組(P<0.05)；③ UC組中,活動期患者血清IDO、KYN水平高②緩解期(P<0.01),併隨炎癥嚴重程度升高而升高；④活動期UC組IDO、KYN水平與疾病活動度、CRP水平呈正相關；⑤ IDO、KYN、CRP的ROC麯線下麵積分彆為0.976、0.856和0.864；最佳截斷值分彆為53.66 U/L、2.34 nmol/L 和1.75 mg/ml；靈敏度分彆為90.5%、78.65%和100.0%；特異度分彆為94.4%、83.3%和61.1%；Youden指數分彆為0.8492、0.6190和0.6111。結論UC患者血清IDO和KYN水平可以作為判斷UC患者炎癥程度的重要指標,血清IDO、KYN和CRP水平對活動期UC有重要的診斷價值,其中 IDO 診斷價值高② KYN 和CRP。
목적검측신타알2,3-쌍가양매(IDO)화견뇨안산(KYN)재궤양성결장염(UC)환자중적표체수평,이용수시자공작특정(ROC)곡선분석혈청IDO、KYN진단활동기UC적개치。방법60례UC조、18례질병대조조화20례건강대조조。채용면역조화법검측UC조결장점막중IDO적표체,ELISA법검측3조혈청중 IDO、KYN 화 C 반응단백(CRP)수평,이용ROC곡선진행분석。결과① UC조혈청중IDO화KYN수평여결장점막IDO표체량정정상관(P<0.05)；② UC조환자혈청IDO급KYN수평고②질병대조조화건강대조조(P<0.05)；③ UC조중,활동기환자혈청IDO、KYN수평고②완해기(P<0.01),병수염증엄중정도승고이승고；④활동기UC조IDO、KYN수평여질병활동도、CRP수평정정상관；⑤ IDO、KYN、CRP적ROC곡선하면적분별위0.976、0.856화0.864；최가절단치분별위53.66 U/L、2.34 nmol/L 화1.75 mg/ml；령민도분별위90.5%、78.65%화100.0%；특이도분별위94.4%、83.3%화61.1%；Youden지수분별위0.8492、0.6190화0.6111。결론UC환자혈청IDO화KYN수평가이작위판단UC환자염증정도적중요지표,혈청IDO、KYN화CRP수평대활동기UC유중요적진단개치,기중 IDO 진단개치고② KYN 화CRP。
Objective Detecting the level of IDO and KYN in ulcerative colitis (UC) patients; using ROC curve to discuss their value in the diagnosis of patients with active UC. Methods 60 cases of UC group, 18 cases of dis-ease control group and 20 cases of healthy control group were included in the study. Immunohistochemical staining methods were used to detect the expression of IDO in colonic mucosa of UC, enzyme-linked immunosorbent (ELISA) methods were used to detect the serum levels of IDO, KYN and CRP, and the value with ROC curve method was analyzed. Results ① There was a positive correlation between IDO level and IDO expression (P<0.05). There was a positive correlation between KYN level and IDO expression (P<0.05). ②The levels of IDO and KYN in UC group were higher than those of disease control group and health control group (P<0.05). ③The levels of IDO and KYN in patients with active UC were significantly higher than that in patients with catabasis UC (P<0.01), and the levels increased with severity of inflammation. ④ The levels of IDO and KYN in active UC were positively related to disease activity and CRP levels (P<0.05). ⑤The IDO,KYN and CRP area under ROC curve were 0.976, 0.856 and 0.864;best cut-off point were 53.66 U/L, 2.34 nmol/L and 1.75 mg/ml;sensitiv-ity were 90.5%, 78.65% and 100.0%; specifity were 94.4%, 83.3% and 61.1%; Youden index were 0.839 2, 0.619 0 and 0.611 1. Conclusion The levels of IDO and KYN in serum can be used as an important index to judge the severity of UC. They have important value in the diagnosis of active UC . The value of IDO is higher than KYN and CRP.
