安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2013年
12期
1480-1483
,共4页
莫碧文%李劳冬%于会娜%王昌明%曾锦荣%李党育%王绩英%韦江红%陈峰%黄剑伟
莫碧文%李勞鼕%于會娜%王昌明%曾錦榮%李黨育%王績英%韋江紅%陳峰%黃劍偉
막벽문%리로동%우회나%왕창명%증금영%리당육%왕적영%위강홍%진봉%황검위
肺癌%Musashi2%干细胞标志物%肿瘤干细胞%纤维支气管镜
肺癌%Musashi2%榦細胞標誌物%腫瘤榦細胞%纖維支氣管鏡
폐암%Musashi2%간세포표지물%종류간세포%섬유지기관경
lung cancer%Musashi2%stem cells marker%cancer stem cells%bronchoscope
目的探讨干细胞标志物Musashi2在肺癌中的表达及其临床意义。方法采用RT-PCR法检测Musashi2 mR-NA在112例肺癌纤支镜活检组织和18例良性肺病变纤支镜活检组织中表达的差异；随后利用免疫组化法验证Mu-sashi2蛋白在50例肺癌组织、32例良性病变肺组织和18例癌旁正常肺组织间表达的差异,并分析 Musashi2 mRNA和蛋白表达与肺癌临床病理参数的关系。结果RT-PCR法检测结果显示,Musashi2 mRNA在肺癌组织中的表达显著高于良性肺病变肺组织(P<0.05)。免疫组化法检测发现,Mu-sashi2蛋白在肺癌组织中的表达显著高于良性病变肺组织和癌旁正常肺组织(P<0.05),与mRNA表达相一致。肺癌组织中Musashi2 mRNA和蛋白的表达与患者年龄、性别、淋巴结转移、TNM分期和肿瘤分化程度均无关。 Musashi2 mR-NA在肺癌中的表达与组织病理类型相关,小细胞肺癌Mu-sashi2 mRNA的表达显著高于肺鳞癌( P <0.05)。结论 Musashi2 mRNA和蛋白在肺癌中高表达,可能参与了肺癌的发生发展。
目的探討榦細胞標誌物Musashi2在肺癌中的錶達及其臨床意義。方法採用RT-PCR法檢測Musashi2 mR-NA在112例肺癌纖支鏡活檢組織和18例良性肺病變纖支鏡活檢組織中錶達的差異；隨後利用免疫組化法驗證Mu-sashi2蛋白在50例肺癌組織、32例良性病變肺組織和18例癌徬正常肺組織間錶達的差異,併分析 Musashi2 mRNA和蛋白錶達與肺癌臨床病理參數的關繫。結果RT-PCR法檢測結果顯示,Musashi2 mRNA在肺癌組織中的錶達顯著高于良性肺病變肺組織(P<0.05)。免疫組化法檢測髮現,Mu-sashi2蛋白在肺癌組織中的錶達顯著高于良性病變肺組織和癌徬正常肺組織(P<0.05),與mRNA錶達相一緻。肺癌組織中Musashi2 mRNA和蛋白的錶達與患者年齡、性彆、淋巴結轉移、TNM分期和腫瘤分化程度均無關。 Musashi2 mR-NA在肺癌中的錶達與組織病理類型相關,小細胞肺癌Mu-sashi2 mRNA的錶達顯著高于肺鱗癌( P <0.05)。結論 Musashi2 mRNA和蛋白在肺癌中高錶達,可能參與瞭肺癌的髮生髮展。
목적탐토간세포표지물Musashi2재폐암중적표체급기림상의의。방법채용RT-PCR법검측Musashi2 mR-NA재112례폐암섬지경활검조직화18례량성폐병변섬지경활검조직중표체적차이；수후이용면역조화법험증Mu-sashi2단백재50례폐암조직、32례량성병변폐조직화18례암방정상폐조직간표체적차이,병분석 Musashi2 mRNA화단백표체여폐암림상병리삼수적관계。결과RT-PCR법검측결과현시,Musashi2 mRNA재폐암조직중적표체현저고우량성폐병변폐조직(P<0.05)。면역조화법검측발현,Mu-sashi2단백재폐암조직중적표체현저고우량성병변폐조직화암방정상폐조직(P<0.05),여mRNA표체상일치。폐암조직중Musashi2 mRNA화단백적표체여환자년령、성별、림파결전이、TNM분기화종류분화정도균무관。 Musashi2 mR-NA재폐암중적표체여조직병리류형상관,소세포폐암Mu-sashi2 mRNA적표체현저고우폐린암( P <0.05)。결론 Musashi2 mRNA화단백재폐암중고표체,가능삼여료폐암적발생발전。
Objective To detect the expression of Musashi2 in lung cancer and to analyze its clinical significance. Methods The differential expression of Musashi2 mRNA was detected using RT-PCR from 112 human lung cancer and 18 non-cancer lung tissues obtained by bronchoscopy. The differential expression of Musashi2 protein was veri-fied using immunohistochemistry from 50 cases lung cancer tissues, 32 cases benign lesion lung tissues and 18cases non-cancer adjacent normal lung tissues. Then the relationships between Musashi2 mRNA and protein expression level and lung cancer clinicopathological factors were analyzed. Results The expression of Musashi2 mRNA and protein in lung cancer tissues were significantly higher than those in non-cancer lung tissues (P<0.05). The ex-pression of Musashi2 mRNA and protein were not correlated with age, gender, lymph node metastasis, TNM stage and differentiation of lung cancer patients. The expression of Musashi2 mRNA was significantly correlated with the histology, which in small cell lung carcinomas was significantly higher than that in squamous cell carcinomas (P<0.05). Conclusion The Musashi2 gene is abnormally upregulated in lung cancer, and might play a crucial role in the carcinogenesis and progression of lung cancer.
