CAJ | 학술논문

目的：观察来氟米特活性代谢产物A771726对脂多糖诱导下HaCaT细胞增殖和凋亡的影响。方法采用MTT法检测细胞增殖水平的变化；流式细胞仪检测细胞凋亡；倒置相差显微镜下观察细胞形态。结果 A771726对脂多糖诱导下HaCaT细胞的增殖有明显的抑制作用，具有时间-浓度依赖性；A771726能促进细胞凋亡，凋亡率随浓度的增加而增加；倒置显微镜下观察，脂多糖组细胞呈致密单层，随着A771726浓度升高，细胞体积变小、细胞间隙变大、细胞内空泡增多。结论来氟米特可以抑制脂多糖诱导下HaCaT细胞增殖，促进其凋亡，这可能是来氟米特治疗银屑病的作用机制之一。
목적：관찰래불미특활성대사산물A771726대지다당유도하HaCaT세포증식화조망적영향。방법채용MTT법검측세포증식수평적변화；류식세포의검측세포조망；도치상차현미경하관찰세포형태。결과 A771726대지다당유도하HaCaT세포적증식유명현적억제작용，구유시간-농도의뢰성；A771726능촉진세포조망，조망솔수농도적증가이증가；도치현미경하관찰，지다당조세포정치밀단층，수착A771726농도승고，세포체적변소、세포간극변대、세포내공포증다。결론래불미특가이억제지다당유도하HaCaT세포증식，촉진기조망，저가능시래불미특치료은설병적작용궤제지일。
Objective To observe the proliferation and apoptosis of the lipopolysaccharide (LPS)-induced HaCaT cells in the presence of the active leflunomide metabolite A 771726 .Methods MTT was used to detect the level of cell proliferation;flow cytometry ( FCM) was used to detect apoptosis of HaCaT cells;inverted phase contrast microscope (IPCM) was used to observe morphologic changes .Results The A771726 significantly inhibited the pro-liferation of LPS-induced HaCaT cells , the inhibition effect was concentration-and-time dependent;the A771726 pro-moted cell apoptosis and the apoptosis rate increased with its concentration , the LPS group showed dense monolayer cells under an inverted microscope , as the concentration of the A 771726 increased , the volume of the cells got smal-ler and intercellular space got larger , and the more vacuoles was observed in the cells .Conclusion Leflunomide can inhibit the proliferation of the LPS induced HaCaT cells and expedite their apoptosis , which may be one of the mecha-nisms of leflunomide in the treatment of psoriasis .