CAJ | 학술논문

　　目的观察外源性脑源性神经营养因子(BDNF)对惊厥持续状态(SC)后海马凋亡调控基因表达的影响。方法选用成年Wistar鼠32只制作氯化锂-匹罗卡品SC模型，另外32只为正常对照组。两组分别于一侧脑室内注射生理盐水、BDNF、抗BDNF抗体或不注射，于注射后6 h处死。免疫组化观察海马Bcl-2和c-Jun蛋白的表达；原位杂交和RT-PCR测定bcl-2和c-jun mRNA的表达。结果正常对照组双侧海马bcl-2及c-jun表达与同组无注射组比较无显著性差异。SC组注射侧海马bcl-2表达由高而低依次为：SC-BDNF组>SC-无注射组=SC-NS组(P<0.05)>SC-抗BDNF组(P<0.05)，c-jun表达则呈相反变化趋势(P<0.05)；SC组注射对侧海马bcl-2及c-jun表达与无注射对照组相比无显著性差异。结论 SC后，脑室内注射外源性BDNF可能通过上调凋亡抑制基因bcl-2、下调凋亡促进基因c-jun表达发挥其保护作用。脑室内注射BDNF在脑内扩散有限，在临床的应用受限。
　　목적관찰외원성뇌원성신경영양인자(BDNF)대량궐지속상태(SC)후해마조망조공기인표체적영향。방법선용성년Wistar서32지제작록화리-필라잡품SC모형，령외32지위정상대조조。량조분별우일측뇌실내주사생리염수、BDNF、항BDNF항체혹불주사，우주사후6 h처사。면역조화관찰해마Bcl-2화c-Jun단백적표체；원위잡교화RT-PCR측정bcl-2화c-jun mRNA적표체。결과정상대조조쌍측해마bcl-2급c-jun표체여동조무주사조비교무현저성차이。SC조주사측해마bcl-2표체유고이저의차위：SC-BDNF조>SC-무주사조=SC-NS조(P<0.05)>SC-항BDNF조(P<0.05)，c-jun표체칙정상반변화추세(P<0.05)；SC조주사대측해마bcl-2급c-jun표체여무주사대조조상비무현저성차이。결론 SC후，뇌실내주사외원성BDNF가능통과상조조망억제기인bcl-2、하조조망촉진기인c-jun표체발휘기보호작용。뇌실내주사BDNF재뇌내확산유한，재림상적응용수한。
Objective To explore the effects of brain-derived neurotrophic factor (BDNF) on expression of apoptotic regulated genes (bcl-2 and c-jun) in hippocampus after status convulsivus (SC). Methods Seizures were induced in 32 adult Wistar rats with lithium-pilocar-pine intraperitoneal injection (SC), the other 32 rats were as the normal controls (NC). The rats were sacrificed 6 h after injection of normal saline (NS), BDNF, or anti-BDNF in left lateral ventricle (or no injection). The expression of Bcl-2 and c-Jun protein and bcl-2 and c-jun mRNA were investigated with immunocytochemistry, RT-PCR and in situ hybridization. Results The expression of bcl-2 and c-jun (both protein and mRNA) was not significantly different in the hippocampus of both side in NC. In SC, the expression of bcl-2 ranged from more to less as BDNF>NS and non-injection>anti-BDNF in the hippocampus of non-injected side, while the expression of c-jun reversed. Howev-er, there was not significant difference in the expression of bcl-2 or c-jun in the hippocampus of non-injected side. Conclusion Exogenous BDNF can up-regulate the expression of bcl-2 and down-regulate the expression of c-jun in hippocampal cells, which may protect brain from apoptosis after after SC. The intraventricular injection of BDNF diffuses limited, which works less for clinical treatment.