经济动物学报
經濟動物學報
경제동물학보
JOURNAL OF ECONOMIC ANIMAL
2012年
1期
35-37
,共3页
貉%GAPDH甘油醛-3-磷酸脱氢酶%克隆
貉%GAPDH甘油醛-3-燐痠脫氫酶%剋隆
맥%GAPDH감유철-3-린산탈경매%극륭
raccoon dog%GAPDH%clone
根据哺乳动物的甘油醛-3-磷酸脱氢酶（glycera ldehyde-3-phosphate dehydrogenase,GAPDH）氨基酸的保守序列,参考犬的GAPDH基因序列,设计一对特异性引物,采用RT-PCR技术,从貉的肝脏中扩增出一段长271 bp的序列。对其序列分析表明：该基因序列与GenBank中已发表的犬的GAPDH基因（GenBank序列号：AB028142,AB038240）同源性为100%。本试验成功地克隆了一段貉GAPDH基因,证实了貉亦存在GAPDH基因且高度保守。
根據哺乳動物的甘油醛-3-燐痠脫氫酶（glycera ldehyde-3-phosphate dehydrogenase,GAPDH）氨基痠的保守序列,參攷犬的GAPDH基因序列,設計一對特異性引物,採用RT-PCR技術,從貉的肝髒中擴增齣一段長271 bp的序列。對其序列分析錶明：該基因序列與GenBank中已髮錶的犬的GAPDH基因（GenBank序列號：AB028142,AB038240）同源性為100%。本試驗成功地剋隆瞭一段貉GAPDH基因,證實瞭貉亦存在GAPDH基因且高度保守。
근거포유동물적감유철-3-린산탈경매（glycera ldehyde-3-phosphate dehydrogenase,GAPDH）안기산적보수서렬,삼고견적GAPDH기인서렬,설계일대특이성인물,채용RT-PCR기술,종맥적간장중확증출일단장271 bp적서렬。대기서렬분석표명：해기인서렬여GenBank중이발표적견적GAPDH기인（GenBank서렬호：AB028142,AB038240）동원성위100%。본시험성공지극륭료일단맥GAPDH기인,증실료맥역존재GAPDH기인차고도보수。
The Raccoon dog GAPDH gene was amplified and cloned by RT-PCR from its liver.The gene was sequenced by 271 bp.Compared with the genes of canine（GenBank numbers：AB028142,AB038240）,they shared 100% homology.This trial proved that the GAPDH gene was existed and conserved in raccoon dog.
