CAJ | 학술논문

目的：了解小窝蛋白-1（ Cav-1）在食管癌患者血清中的表达情况，探讨Cav-1是否可以作为食管癌肿瘤标记物。方法⑴用Western Blot方法检测食管癌细胞EC109、EC9706中Cav-1的表达情况；⑵收集本院43例食管癌患者血清，30例良性食管病变患者血清及50例健康体检者血清，应用ELISA方法检测其Cav-1的表达情况。结果⑴Western Blot 结果显示，Cav-1在食管癌细胞EC109、EC9706中均表达；在低分化的食管癌细胞EC109中表达水平明显高于高分化的食管癌细胞EC9706（ t ＝3.08， P ＝0.035）。⑵ELISA结果显示，食管癌患者血清Cav-1中位数为0.104 ng／ml；良性食管病变患者血清Cav-1中位数为0.075 ng／ml；健康体检者血清Cav-1中位数为0.081 ng／ml；食管癌患者血清与良性食管病变及健康体检者对比，差异有统计学意义（χ2＝23.16， P ＝0.001）。结论 Cav-1在食管癌患者血清中的表达高于良性食管病变患者及健康体检者，有望成为食管癌肿瘤标志物。
목적：료해소와단백-1（ Cav-1）재식관암환자혈청중적표체정황，탐토Cav-1시부가이작위식관암종류표기물。방법⑴용Western Blot방법검측식관암세포EC109、EC9706중Cav-1적표체정황；⑵수집본원43례식관암환자혈청，30례량성식관병변환자혈청급50례건강체검자혈청，응용ELISA방법검측기Cav-1적표체정황。결과⑴Western Blot 결과현시，Cav-1재식관암세포EC109、EC9706중균표체；재저분화적식관암세포EC109중표체수평명현고우고분화적식관암세포EC9706（ t ＝3.08， P ＝0.035）。⑵ELISA결과현시，식관암환자혈청Cav-1중위수위0.104 ng／ml；량성식관병변환자혈청Cav-1중위수위0.075 ng／ml；건강체검자혈청Cav-1중위수위0.081 ng／ml；식관암환자혈청여량성식관병변급건강체검자대비，차이유통계학의의（χ2＝23.16， P ＝0.001）。결론 Cav-1재식관암환자혈청중적표체고우량성식관병변환자급건강체검자，유망성위식관암종류표지물。
Objective To investigate the expression of caveolin-1 in the sera of esophageal cancer patients , and the possibility of caveolin-1 to be the tumor marker of an esophageal cancer .Methods ⑴Western blot was used to detect the expression of caveolin-1 in esophageal cancer cell EC 109 and EC9706;⑵The sera were collected from 43 esophageal cancer patients , 30 benign esophageal lesions, and 50 healthy control men.Enzyme linked immunosorbent assay (ELISA) was used to detect caveolin-1 in serum.Results⑴Western blot showed that caveolin-1 was detected in two types of esophageal cancer cell lines .The level of caveolin-1 in the poor dif-ferentiated EC109 cell line was mush higher than in the well differentiated EC 9706 cell line ( t =3.08 , P =0.035 ) .⑵ ELISA re-vealed that the median level of serum caveolin-1 in 43 esophageal cancers (0.104 ng/ml) was significantly higher than 30 benign e-sophageal lesions (0.075 ng/ml) or 50 healthy control men (0.081 ng/ml) (χ2 =23.16, P =0.001).Conclusions Caveolin-1 may be the tumor marker of an esophageal cancer as its level in esophageal patients was higher than in benign esophageal lesions pa -tients or healthy control men .