CAJ | 학술논문

海绵相关微生物是药物开发的重要资源．本文利用6种培养基对汕头南澳普遍存在的海绵Halichondriasp．的共附生放线菌进行分离和培养．共得到36株放线菌．基于16SrRNA基因序列的系统发育分析表明．这些放线菌分别属于Streptomyces、Nocardiopsis和Mi．cromonospora属．抗菌活性筛选结果显示，分离菌株分别对Staphylococcus aureus、Bacillussubtilis和EscherichincDli等的生长有抑制作用．同时分子方法筛选显示这些放线菌具有潜在产生聚酮类、角蒽环类和非核糖体多肽类的能力．本研究结果为海绵相关微生物在药物开发方面积累了基础数据．
해면상관미생물시약물개발적중요자원．본문이용6충배양기대산두남오보편존재적해면Halichondriasp．적공부생방선균진행분리화배양．공득도36주방선균．기우16SrRNA기인서렬적계통발육분석표명．저사방선균분별속우Streptomyces、Nocardiopsis화Mi．cromonospora속．항균활성사선결과현시，분리균주분별대Staphylococcus aureus、Bacillussubtilis화EscherichincDli등적생장유억제작용．동시분자방법사선현시저사방선균구유잠재산생취동류、각은배류화비핵당체다태류적능력．본연구결과위해면상관미생물재약물개발방면적루료기출수거．
In this study, actinobacteria isolated fi＇om the marine sponge Ha!ichondria sp. collected in Shantou Nan＇ao of the South China Sea are described. A total of 36 actinobacteria were isolated by 6 different media. The 16S rRNA genes of 36 isolates were cloned and the sequence results showed that the isolates belong to the genera Streptomyces, Nocardiopsis and Micromonospora. All the isolates were screened for antimicrobial actives and genes encoding polyketide synthases（PKS）, angucycline and nonribosomal peptide synthetases（NRPS）. The results show that almost all isolates have PKS, angucycline, NRPS genes and antimicrobial activties in indicators of Staphylococcus aureus, Bacillus subtilis and Escherichia coll.