泉州师范学院学报
泉州師範學院學報
천주사범학원학보
JOURNAL OF QUANZHOU NORMAL COLLGEG
2012年
2期
67-71
,共5页
麻竹%龙竹%FT同源基因%PCR扩增%序列分析
痳竹%龍竹%FT同源基因%PCR擴增%序列分析
마죽%룡죽%FT동원기인%PCR확증%서렬분석
dendrocalamus latiflorus%dendrocalamus giganteus%FT-like gene%amplification%sequence analysis
根据拟南芥Flowering Locus T(FT)基因和水稻Heading date 3a(Hd3a)基因的序列设计引物,通过PCR扩增的方法分别从麻竹和龙竹基因组DNA中各得到长为334bp的DNA片段.经BLAST检索、基因结构分析和编码蛋白功能域预测等分析,初步确定所得DNA片段可能是麻竹和龙竹中的FT同源基因的部分序列.
根據擬南芥Flowering Locus T(FT)基因和水稻Heading date 3a(Hd3a)基因的序列設計引物,通過PCR擴增的方法分彆從痳竹和龍竹基因組DNA中各得到長為334bp的DNA片段.經BLAST檢索、基因結構分析和編碼蛋白功能域預測等分析,初步確定所得DNA片段可能是痳竹和龍竹中的FT同源基因的部分序列.
근거의남개Flowering Locus T(FT)기인화수도Heading date 3a(Hd3a)기인적서렬설계인물,통과PCR확증적방법분별종마죽화룡죽기인조DNA중각득도장위334bp적DNA편단.경BLAST검색、기인결구분석화편마단백공능역예측등분석,초보학정소득DNA편단가능시마죽화룡죽중적FT동원기인적부분서렬.
In this paper,primers were designed according to the conserved exons of Arabidopsis thaliana Flowering Locus T(FT) gene and Oryza sativa Heading date 3a(Hd3a) gene to amplify the corresponding DNA sequence from genomic DNA of D.latiflorus and D.giganteus,and two 334 bp long DNA sequences were obtained.Through blast search,gene structure analysis and conserved domain search,we conclud that these two DNA sequences might be members of FT gene family in D.latiflorus and D.giganteus.