CAJ | 학술논문

为了得到一种效果较好的测定蜂花粉中总维生素C含量的方法,本文采用高效液相色谱法对油菜蜂花粉进行总VC的定性与定量分析,从结果的稳定性、精密度和重现性三方面对其进行了考量.结果发现,总VC含量在12.70～200.00μg范围内与色谱峰面积呈良好的线性关系,且回收率在95.71%～98.60%,相对标准偏差为1.05%;稳定性实验的相对标准偏差为2.12%,精密度实验的相对标准偏差为1.1%;最终测得油菜蜂花粉样品的总VC平均含量为36.99 mg.（100g）-1,相对标准偏差为1.43%.本研究所建立的HPLC测定蜂花粉中总VC含量的方法简单快速、重现性好,可以较为准确的实现定性与定量分析.
위료득도일충효과교호적측정봉화분중총유생소C함량적방법,본문채용고효액상색보법대유채봉화분진행총VC적정성여정량분석,종결과적은정성、정밀도화중현성삼방면대기진행료고량.결과발현,총VC함량재12.70～200.00μg범위내여색보봉면적정량호적선성관계,차회수솔재95.71%～98.60%,상대표준편차위1.05%;은정성실험적상대표준편차위2.12%,정밀도실험적상대표준편차위1.1%;최종측득유채봉화분양품적총VC평균함량위36.99 mg.（100g）-1,상대표준편차위1.43%.본연구소건립적HPLC측정봉화분중총VC함량적방법간단쾌속、중현성호,가이교위준학적실현정성여정량분석.
To obtain an efficient method for determination of total vitamin C（VC） in bee pollen,high-performance liquid chromatography（HPLC） was employed and investigated for its stability,precision and repeatability in qualitative and quantitative analysis of total VC in bee pollen.As the results,total VC content and chromatogram peak area presented good linear correlation.The recoveries were 95.71%～98.60% with RSD 1.05%.The RSD of the stability and precision experiments were 2.12% and 1.1% respectively.Total VC content in rape bee pollen was 36.99 mg/100 g in average,and its RSD was 1.43%.In conclusion,HPLC can be used as a simple and fast method for qualitative and quantitative analysis of total VC in bee pollen.