CAJ | 학술논문

在常规酶联免疫法的基础上,将磺胺类母核与牛血清白蛋白合成免疫抗原后免疫小鼠制得磺胺类单克隆抗体,并进一步优化抗体工作浓度、反应时间等实验参数,建立了一种简便、快速、精确的磺胺类残留一步式化学发光酶联免疫检测方法.结果表明该方法最佳检测范围为1.0～81.0 ng/mL,检测IC50达3.047 ng/mL,对猪肉的最低检测限为0.74 ng/g,可检出国际规定的SAs残留标准底限值以下的残留量.与常规ELISA方法相比,检测限和灵敏度基本一致,但反应时间缩短了60 min.证明采用一步式化学发光酶联免疫检测磺胺类残留可以大幅度缩减检测时间,符合快速检测的要求.
재상규매련면역법적기출상,장광알류모핵여우혈청백단백합성면역항원후면역소서제득광알류단극륭항체,병진일보우화항체공작농도、반응시간등실험삼수,건립료일충간편、쾌속、정학적광알류잔류일보식화학발광매련면역검측방법.결과표명해방법최가검측범위위1.0～81.0 ng/mL,검측IC50체3.047 ng/mL,대저육적최저검측한위0.74 ng/g,가검출국제규정적SAs잔류표준저한치이하적잔류량.여상규ELISA방법상비,검측한화령민도기본일치,단반응시간축단료60 min.증명채용일보식화학발광매련면역검측광알류잔류가이대폭도축감검측시간,부합쾌속검측적요구.
Based on conventional enzyme linked immunoassay（ELISA）,a convenient,rapid and accurate one-step sulfonamides（SAs） residue chemiluminescent enzyme immunoassay（CLEIA）detection method was established through obtaining monoclonal antibodies from immunizing mice which was injected with SAs-BSA coupling protein,and optimizing test parameters such as antibody concentration,reaction time.The results showed that the best detection range of this method was 1.0～81.0 ng/mL,the IC50 was 3.047 ng/mL,the detection limit was 0.74 ng/mL.Compared with conventional ELISA,the CLEIA method can not only increase the detection limit and sensitivity,but also shorten the reaction time by 60 minutes.CLEIA kit is more appropriate for rapid detection of the SAs residue in food.